Gt(ROSA)26Sor<tm1(tTA)Roos> Targeted Allele Detail MGI Mouse (MGI:3806275)

Gt(ROSA)26Sor^tm1(tTA)Roos
Targeted Allele Detail

Nomenclature

Symbol:	Gt(ROSA)26Sor^tm1(tTA)Roos
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Raymond Roos
MGI ID:	MGI:3806275
Synonyms:	ROSA:LNL:tTA
Gene:	Gt(ROSA)26Sor Location: unknown Genetic Position: Chr6, 48.7 cM

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:138761
Parent Cell Line:	HM-1 (ES Cell)
Strain of Origin:	129P2/OlaHsd-Hprt1^b-m3

Mutation
description

Allele Type:	Targeted (Floxed/Frt)
Mutation:	Insertion
	A targeting vector was designed to insert a loxP-flanked STOP cassette (containing a neomycin resistance cassette and transcriptional STOP element) just upstream of a modified/optimized form of tetracycline controlled transactivator ("mtTA") into the Gt(ROSA)26Sor locus. and chimeric mice were established. (J:138761)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	90 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2
growth/size		N	√
growth/size phenotype		N
decreased body weight			√
		tg1	tg2
nervous system		N	√
nervous system phenotype		N	N
decreased motor neuron number			√
abnormal ventral spinal root morphology			√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Gt(ROSA)26Sor^tm1(tTA)Roos/? Lhx3^tm1(cre)Slp/Lhx3⁺ Tg(tetO-SOD1,-luc)1Roos/0	involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
nervous system nervous system phenotype (J:138761) mice show no significant differences from triple mutants carrying the G93A version of SOD1, in the rotarod test when tested at 300 days and later growth/size growth/size phenotype (J:138761) body weight is similar to non-transgenic controls
Genotype	Allelic Composition	Genetic Background
tg2	Gt(ROSA)26Sor^tm1(tTA)Roos/? Lhx3^tm1(cre)Slp/Lhx3⁺ Tg(tetO-SOD1*G93A,-luc)1Roos/0	involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
nervous system nervous system phenotype (J:138761) mice show no significant differences relative to triple mutants carrying wild-type human SOD1, in the rotarod test when tested at 300 days and later decreased motor neuron number (J:138761) significant decrease in number of spinal motor neurons (MNs) compared to wild-type and transgenic controls is observed at 450 days of age abnormal ventral spinal root morphology (J:138761) significant decrease in number of axons in L5 anterior root at 450 days is observed compared to triple mutants expressing wild-type human SOD1 growth/size decreased body weight (J:138761) after 300 days, mean body weight is reduced relative to wild-type or to triple mutants carrying the wild-type human SOD1 gene

References

Original:	J:138761 Wang L et al., "Restricted expression of mutant SOD1 in spinal motor neurons and interneurons induces motor neuron pathology." Neurobiol Dis 2008 Mar;29(3):400-8
All:	1 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources Funding Information Warranty Disclaimer & Copyright Notice Send questions and comments to User Support.	last database update 11/20/2009 MGI_4.31 Web browser compatibility