|
|
| Nomenclature |
|
Symbol:
|
Chattm1(cre/Esr1)Nat
|
|
Name:
|
choline acetyltransferase;
targeted mutation 1, Jeremy Nathans
|
|
MGI ID: |
MGI:3777637 |
|
Synonyms: |
ChAT-IRES-creER(T) |
|
Gene:
|
Chat
Location:
Chr14:33221389-33279095 bp, - strand
Genetic Position: Chr14,
10.5 cM
|
|
Mutation
origin |
|
Germline Transmission:
| Earliest citation of germline transmission:
J:144315
|
|
Parent Cell Line:
| Other (see notes) (ES Cell) |
|
Strain of Origin:
|
129
|
|
Mutation
description |
|
Allele
Type: |
Targeted (knock-in) |
|
Mutation: |
Insertion |
| |
An IRES-creER cassette, a tetracycline resistance gene, and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, cre expression was detected in amacrine cells of the retina, and in a sparse number of cholinergic neurons in the brain. (J:144315) |
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
Recombinase specificity
|
|
Phenotype
summary
|
|
Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
|
Key:
|
| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
 |
expected model not found |
|
|
|
|
|
Phenotypic
data by
genotype
|
|
Phenotypic Data by Genotype
(show or
hide all phenotypic details)
|
|
|
|
|
Notes |
ES cell line = JHMI-ES1.
|
| References |
|
Original: |
J:144315
Rotolo T et al.,
"Genetically-directed, cell type-specific sparse labeling for the analysis of neuronal morphology."
PLoS ONE 2008;3(12):e4099
|
|
All: |
1 reference(s)
|
|
