Tg(tetO-Clock<m1Jt>)CL57Jt Transgene Detail MGI Mouse (MGI:3772924)

Tg(tetO-Clock^m1Jt)CL57Jt
Transgene Detail

Nomenclature

Symbol:	Tg(tetO-Clock^m1Jt)CL57Jt
Name:	transgene insertion CL57, Joseph S Takahashi
MGI ID:	MGI:3772924
Synonyms:	tetO::Clock^{delta 19}
Transgene:	Tg(tetO-Clock^m1Jt)CL57Jt Location: unknown

Transgene
origin

Strain of Origin:

CD-1

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	A transgenic vector was designed to carry the full-length Clock mutant gene downstream of a tetracycline-responsive promoter (TRE; tetO). Crossing these mice with mice expressing the tTA or reverse tetracycline-controlled transactivator protein under control of tissue-specific promoters, transgene expression can be regulated in appropriate tissues in the offspring with administration of the tet analog doxycycline. (J:120326)

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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

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Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1
behavior/neurological		√
prolonged circadian period		√
abnormal circadian phase		√
delayed circadian phase		√
abnormal locomotor activity		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Tg(Scg2-tTA)1Jt/0 Tg(tetO-Clock^m1Jt)CL57Jt/0	involves: C57BL/6J * CD-1
behavior/neurological prolonged circadian period (J:120326) when released into constant darkness, mice show a lengthened circadian period about 1 hour greater than wild-type when treated with doxycycline (2 mg/ml) in drinking water, circadian period is shortened within one day to 23.7 hours, not significantly different from wild-type period of 23.6 hours when these mice are treated with doxycycline for 3 weeks before removal, lengthening of circadian period is not immediate, but takes longer than 3 months to lengthen to the previously observed increased length treatment with 10 ug/ml doxycycline immediately switches off transgene expression, but reversal upon return to normal water is rapid, occurring within 2-3 days rather than >3 months at 2 mg/ml doxycycline; 100 ug/ml doxycycline is as effective in regulating transgene expression abnormal circadian phase (J:120326) transgenic mice show significant effects on phase resetting and period lengthening effects on locomotor activity rhythms delayed circadian phase (J:120326) when given a 6 hour light pulse during subjective night (circadian time (CT) 12 and 17), mice exhibit a significantly larger phase delay when the transgenes are being expressed relative to delay displayed when under doxycycline suppression of transgene expression phase shift is larger when transgene expression is on compared to when expression is suppressed abnormal locomotor activity (J:120326) with transgene expression, mice display lengthening of locomotor activity rhythms

References

Original:	J:120326 Hong HK et al., "Inducible and reversible Clock gene expression in brain using the tTA system for the study of circadian behavior." PLoS Genet 2007 Feb 23;3(2):e33
All:	1 reference(s)

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