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| Nomenclature |
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Symbol:
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Pou5f1tm1Jae
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Name:
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POU domain, class 5, transcription factor 1;
targeted mutation 1, Rudolf Jaenisch
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MGI ID: |
MGI:3771368 |
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Synonyms: |
Oct4-IRES-GFPneo, Oct4-neo |
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Gene:
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Pou5f1
Location:
Chr17:35643007-35647714 bp, + strand
Genetic Position: Chr17,
19.23 cM
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Mutation
origin |
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Germline Transmission:
| Earliest citation of germline transmission:
J:130250
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Parent Cell Line:
| v6.5 (ES Cell) |
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Strain of Origin:
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(C57BL/6 x 129S4/SvJae)F1
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Mutation
description |
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Allele
Type: |
Targeted (Reporter) |
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Mutation: |
Insertion |
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The Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. (J:130250) |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
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Key:
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| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
 |
expected model not found |
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Phenotypic
data by
genotype
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Phenotypic Data by Genotype
(show or
hide all phenotypic details)
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Notes |
When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts.
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| References |
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Original: |
J:130250
Wernig M et al.,
"In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state."
Nature 2007 Jul 19;448(7151):318-24
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All: |
2 reference(s)
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