Tg(Alb-PLG)1Dgi Transgene Detail MGI Mouse (MGI:3763545)

Tg(Alb-PLG)1Dgi
Transgene Detail

Nomenclature

Symbol:	Tg(Alb-PLG)1Dgi
Name:	transgene insertion 1, David Ginsburg
MGI ID:	MGI:3763545
Transgene:	Tg(Alb-PLG)1Dgi Location: unknown

Transgene
origin

Strain of Origin:

(C57BL6/J x SJL/J)F1 X (C57BL6/J x SJL/J)F1 or C57BL/6 X (C57BL6/J x SJL/J)F1

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	A cassette containing the human PLG cDNA from nucleotide -13 (A of ATG initiation codon as +1) to +2476 (including the entire coding sequence) was followed by the green fluorescent protein (EGFP) coding sequence, preceded by an internal ribosome entry site sequence (IRES) and a bacterial kanamycin-resistant gene (KAN). A chimeric intron with optimized splice signals from the pCI expression vector was placed upstream of the PLG cDNA. Homologous recombination between the transgene targeting construct and the murine Alb BAC was carried out in E. coli, replacing the murine Alb gene (from nucleotide -12 to exon 12) by the transgene cassette. EGFP expression is not detected. (J:120780)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2
digestive/alimentary system			√
rectal prolapse			√
		tg1	tg2
growth/size			√
decreased body weight			√
		tg1	tg2
immune system		√
increased susceptibility to bacterial infection		√
		tg1	tg2
life span/aging		√	N
life span/aging phenotype			N
increased sensitivity to induced morbidity/mortality		√
		tg1	tg2
liver/biliary system			N
liver/biliary system phenotype			N

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Tg(Alb-PLG)1Dgi/0	involves: C57BL/6 * C57BL/6J * SJL
life span/aging increased sensitivity to induced morbidity/mortality (J:120780) mice infected subcutaneously with the human pathogenic group A streptococcus (GAS) strain UMAA2616 exhibit 75% of mortality unlike similarly treated wild-type mice that exhibit 20% mortality mice infected subcutaneously with GAS strain MGAS166 exhibit 35% to 100% of mortality unlike similarly treated wild-type mice that exhibit 30% mortality mice infected subcutaneously with PAM-expressing GAS strain AP53 exhibit 80% mortality compared to 20% in similarly treated wild-type mice however, mice exhibit normal mortality when infected with GAS strain UMAA2641, which lacks the Ska gene, or when infected intravenously with UMAA2616 immune system increased susceptibility to bacterial infection (J:120780) mice infected subcutaneously with the human pathogenic group A streptococcus (GAS) strain UMAA2616 exhibit 75% of mortality unlike similarly treated wild-type mice that exhibit 20% mortality mice infected subcutaneously with GAS strain MGAS166 exhibit 35% to 100% of mortality unlike similarly treated wild-type mice that exhibit 30% mortality mice infected subcutaneously with PAM-expressing GAS strain AP53 exhibit 80% mortality compared to 20% in similarly treated wild-type mice mice infected subcutaneously with GAS exhibit increased bacterial colonies in the spleen compared to similarly infected wild-type mice however, mice exhibit normal mortality when infected with GAS strain UMAA2641, which lacks the Ska gene, or when infected intravenously with UMAA2616
Genotype	Allelic Composition	Genetic Background
tg2	Plg^tm1Dco/Plg^tm1Dco Tg(Alb-PLG)1Dgi/0	involves: 129S2/SvPas * C57BL/6 * C57BL/6J * SJL
life span/aging life span-post-weaning/aging (J:120780) LPS-treated mice exhibit normal mortality digestive/alimentary system rectal prolapse (J:120780) mice exhibit a near complete rescue of the rectal prolapse observed in Plg^tm1Dco homozygotes growth/size decreased body weight (J:120780) mice exhibit a near complete rescue of the decreased body weight observed in Plg^tm1Dco homozygotes liver/biliary system liver/biliary system phenotype (J:120780) liver fibrosis observed in Plg^tm1Dco homozygotes is reversed

References

Original:	J:120780 Sun H et al., "Plasminogen is a critical host pathogenicity factor for group A streptococcal infection." Science 2004 Aug 27;305(5688):1283-6
All:	2 reference(s)

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