Tg(KRT14-rtTA)F42Efu Transgene Detail MGI Mouse (MGI:3762564)

Tg(KRT14-rtTA)F42Efu
Transgene Detail

Nomenclature

Symbol:	Tg(KRT14-rtTA)F42Efu
Name:	transgene insertion F42, Elaine Fuchs
MGI ID:	MGI:3762564
Synonyms:	K14rtTA
Transgene:	Tg(KRT14-rtTA)F42Efu Location: unknown

Transgene
origin

Strain of Origin:

FVB

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3' UTR was injected into FVB embryos. (J:56822, J:116057)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2
digestive/alimentary system			√
abnormal tongue epithelium morphology			√
		tg1	tg2
endocrine/exocrine glands			√
abnormal sebaceous gland morphology			√
		tg1	tg2
skin/coat/nails			√
abnormal hair follicle development			√
abnormal hair cycle			√
abnormal skin development			√
abnormal epidermal layer morphology			√
abnormal epidermis stratum spinosum morphology			√
		tg1	tg2
normal phenotype		N
no abnormal phenotype detected		N

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Tg(KRT14-rtTA)F42Efu/0	involves: FVB
normal phenotype no abnormal phenotype detected (J:116057) hemizygotes are viable, fertile, and normal in size; mice do not display any gross physical or behavioral abnormalities
Genotype	Allelic Composition	Genetic Background
tg2	Tg(KRT14-rtTA)F42Efu/0 Tg(tetO-Tcf3)1Efu/0	involves: FVB
skin/coat/nails abnormal hair follicle development (J:116057) after induction of Tcf3 with doxycycline treatment beginning on P1, hair follicle maturation is impaired; follicle downgrowth appears to arrest with Tcf3 induction abnormal hair cycle (J:116057) when skin from doxycycline-treated P transgenic is transplanted onto control mice, by day 27 specified follicles have undergone degeneration and fail to reenter the hair cycle epidermis and residual established hair follicles in grafts have developed into large masses of undifferentiated epithelial cells lacking all markers of epidermal terminal differentiation abnormal skin development (J:116057) epidermal differentiation is severely diminished within 4 days of doxycycline administration (resulting in Tcf3 induction) starting at postnatal day 1 abnormal epidermal layer morphology (J:116057) keratohyalin granules, loricrin, and involucrin are dramatically reduced within 4 days of doxycycline administration starting on P1 flattened squames are present at the skin surface; these appear to be dead keratinocytes abnormal epidermis stratum spinosum morphology (J:116057) within 4 days of doxycycline administration starting on P1, thick filament bundles characteristic of spinous layer K1/K10 filaments are barely detectable endocrine/exocrine glands abnormal sebaceous gland morphology (J:116057) induction of Tcf3 beginning on P1 impairs the end stage of sebaceous gland differentiation; 48 hours after doxycycline treatment, numbers of sebocytes are reduced, and by 4 days, sebocytes are no longer detected digestive/alimentary system abnormal tongue epithelium morphology (J:116057) when pregnant double transgenic mice are administered doxycycline when the embryos are E13.5, epidermal differentiation in the tongue of pups is significantly impaired when examined at P1; the differentiation marker loricrin is reduced in the epithelium, while the simple epithelial marker K8 shows significant induction of expression

References

Original:	J:116057 Nguyen H et al., "Tcf3 governs stem cell features and represses cell fate determination in skin." Cell 2006 Oct 6;127(1):171-83
All:	2 reference(s)

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