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| Nomenclature |
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Symbol:
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Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc
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Name:
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transene insertion 837, Oksana Lockridge
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MGI ID: |
MGI:3720257 |
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Synonyms: |
G117H BChE, M844T |
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Transgene:
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Tg(Gt(ROSA)26Sor-BCHE*G117H)837Loc
Location:
unknown
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Transgene
origin |
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Strain of Origin:
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C57BL/6 x (C57BL/6 x SJL)F1
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Transgene
description |
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Transgene
Type: |
Transgenic (random, expressed) |
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Mutation: |
Insertion |
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The human G117H BChE sequence (encoding the 28 amino acid signal peptide and 574 amino acid full-length protein) was modified with a single His for Gly amino acid mutation at codon 117 (G117H). To enhance expression, a chimeric intron (consisting of a human beta-globin gene and the branch and 3' acceptor site from the intron of an immunoglobulin gene heavy chain variable region) was placed between the promoter and human G117H BChE sequence. A FLAG epitope tag (encoding amino acids DYKDDDDK) was placed at the carboxy-terminus of the human G117H BChE. The transgene also contained the human elongation factor 1-alpha poly(A) sequence at the 3' end. This transgene was microinjected into fertilized [C57BL/6 x (C57BL/6xSJL)F1] eggs. Founder mice (line M837T) were bred to B6SJL/F1Tac mice. All tested tissues express G117H BChE enzyme activity. In addition, 2 other stable lines M844T and M853T were generated, with detailed analysis of M844T described. (J:89332) |
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Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
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Key:
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| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
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expected model not found |
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Phenotypic
data by
genotype
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Phenotypic Data by Genotype
(show or
hide all phenotypic details)
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| References |
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Original: |
J:89332
Wang Y et al.,
"Resistance to organophosphorus agent toxicity in transgenic mice expressing the G117H mutant of human butyrylcholinesterase."
Toxicol Appl Pharmacol 2004 May 1;196(3):356-66
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All: |
1 reference(s)
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