Tg(Scg2-tTA)1Jt Transgene Detail MGI Mouse (MGI:3714754)

Tg(Scg2-tTA)1Jt
Transgene Detail

Nomenclature

Symbol:	Tg(Scg2-tTA)1Jt
Name:	transgene insertion 1, Joseph S Takahashi
MGI ID:	MGI:3714754
Synonyms:	Scg2::tTA
Transgene:	Tg(Scg2-tTA)1Jt Location: unknown

Transgene
origin

Strain of Origin:

CD-1

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	9.85 kb of DNA upstream from the mouse Scg2 translational start site was placed upstream of the tTA gene encoded in the pMMY20 plasmid. The transgene shows expression in the brain and is enriched in the suprachiasmatic nucleus. (J:116189)

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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2	tg3
behavior/neurological		√	√	√
behavior/neurological phenotype		N
abnormal circadian period		√
prolonged circadian period			√
shortened circadian period				√
abnormal circadian phase			√
delayed circadian phase			√
abnormal locomotor activity			√
abnormal locomotor activation		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Arntl^tm1Bra/Arntl^tm1Bra Tg(Scg2-tTA)1Jt/0 Tg(tetO-Arntl)1Jt/0	involves: 129/Sv * C57BL/6J * CD-1
behavior/neurological behavior/neurological phenotype (J:116189) rescued mice exhibit a consistent circadian rhythm (without doxycycline-treatment) which is abolished within 1 to 2 days of doxycycline treatment, then regained after doxycycline withdrawal abnormal circadian period (J:116189) free-running period of rescued mice (bitransgenic null mice without doxycycline treatment) is about 1 hour shorter than in wild-type abnormal locomotor activation (J:116189) although circadian rhythm is restored, level activity and amplitude are significantly lower than observed in wild-type
Genotype	Allelic Composition	Genetic Background
tg2	Tg(Scg2-tTA)1Jt/0 Tg(tetO-Clock^m1Jt)CL57Jt/0	involves: C57BL/6J * CD-1
behavior/neurological prolonged circadian period (J:120326) when released into constant darkness, mice show a lengthened circadian period about 1 hour greater than wild-type when treated with doxycycline (2 mg/ml) in drinking water, circadian period is shortened within one day to 23.7 hours, not significantly different from wild-type period of 23.6 hours when these mice are treated with doxycycline for 3 weeks before removal, lengthening of circadian period is not immediate, but takes longer than 3 months to lengthen to the previously observed increased length treatment with 10 ug/ml doxycycline immediately switches off transgene expression, but reversal upon return to normal water is rapid, occurring within 2-3 days rather than >3 months at 2 mg/ml doxycycline; 100 ug/ml doxycycline is as effective in regulating transgene expression abnormal circadian phase (J:120326) transgenic mice show significant effects on phase resetting and period lengthening effects on locomotor activity rhythms delayed circadian phase (J:120326) when given a 6 hour light pulse during subjective night (circadian time (CT) 12 and 17), mice exhibit a significantly larger phase delay when the transgenes are being expressed relative to delay displayed when under doxycycline suppression of transgene expression phase shift is larger when transgene expression is on compared to when expression is suppressed abnormal locomotor activity (J:120326) with transgene expression, mice display lengthening of locomotor activity rhythms
Genotype	Allelic Composition	Genetic Background
tg3	Tg(Scg2-tTA)1Jt/0 Tg(tetO-Clock)1Jt/0	involves: C57BL/6J * CD-1
behavior/neurological shortened circadian period (J:120326) double transgenic mice display a circadian period that is shortened by 1 hour relative to wild-type mice treatment with doxycycline to suppress transgenic Clock expression demonstrates a rapid switch-off of expression and rapid turn-on upon return to normal water

References

Original:	J:116189 McDearmon EL et al., "Dissecting the functions of the mammalian clock protein BMAL1 by tissue-specific rescue in mice." Science 2006 Nov 24;314(5803):1304-8
All:	2 reference(s)

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