Tg(tetO-Eda*A1)1Dsch Transgene Detail MGI Mouse (MGI:3713107)

Tg(tetO-Eda*A1)1Dsch
Transgene Detail

Nomenclature

Symbol:	Tg(tetO-Eda*A1)1Dsch
Name:	transgene insertion 1, David Schlessinger
MGI ID:	MGI:3713107
Synonyms:	mEDA-A1
Transgene:	Tg(tetO-Eda*A1)1Dsch Location: unknown

Transgene
origin

Strain of Origin:

C57BL/6J

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	This transgene consists of the coding region of the mouse Eda-A1 isoform inserted between TRE/PminCMV promoter and SV40 poly A region. The tetracycline-controlled transactivator (tTA) binds to TRE and activates transcription. Expression of Eda is repressed by the addition of doxicycline and is expressed in doxicycline�s absence. The receptor for the Eda-A1 isoform is Edar. (J:86628)

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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 0 strains available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2
endocrine/exocrine glands		√	√
abnormal gland morphology			√
abnormal sebaceous gland morphology		√	√
abnormal meibomian gland morphology		√
absent meibomian glands			√
abnormal preputial gland morphology			√
enlarged sebaceous gland			√
		tg1	tg2
growth/size			√
decreased body weight			√
		tg1	tg2
immune system			N
immune system phenotype			N
		tg1	tg2
limbs/digits/tail		N
limbs/digits/tail phenotype		N
		tg1	tg2
renal/urinary system			√
abnormal preputial gland morphology			√
		tg1	tg2
reproductive system			√
abnormal preputial gland morphology			√
		tg1	tg2
skin/coat/nails		√	√
skin/coat/nails phenotype		N
abnormal coat/ hair morphology			√
disheveled coat			√
abnormal hair growth			√
short hair			√
abnormal hair types			√
abnormal guard hair		√
decreased zigzag hair amount		√
absent zigzag hairs			√
abnormal skin morphology			√
		tg1	tg2
vision/eye		√	√
abnormal eyelid morphology		√
abnormal meibomian gland morphology		√
absent meibomian glands			√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Tg(MMTVtTA)1Mam/0 Tg(tetO-Eda*A1)1Dsch/0	involves: C57BL/6
endocrine/exocrine glands abnormal sebaceous gland morphology (J:99510) hypertrophy of sebaceous glands associated with hair follicles of the eyelids abnormal meibomian gland morphology (J:86628) enlarged meibomian gland in the absence of doxicycline (transgene is expressed) however, the preputial gland and sweat glands are normal skin/coat/nails skin/coat/nails phenotype (J:86628) in the absence of doxicycline (the transgene is expressed), overall skin morphology is indistinguishable from controls abnormal guard hair (J:86628) in the absence of doxicycline (the transgene is expressed), guard hair numbers are somewhat increased to 8.7% compared to 5-6% in wild-type decreased zigzag hair amount (J:86628) in the absence of doxicycline), zigzag hair is reduced to about 6% which is at least 6-fold less than in wild-type zigzag hair is not restored when the transgene is turned off in adults by the addition of doxicycline, indicating an irreversible role in embryonic stages vision/eye abnormal eyelid morphology (J:99510) eyelids show a ring of yellow hair with slightly thicker skin around the eyelashes than seen in wild-type abnormal meibomian gland morphology (J:86628) enlarged meibomian gland in the absence of doxicycline (transgene is expressed) however, the preputial gland and sweat glands are normal limbs/digits/tail limbs/digits/tail phenotype (J:86628) in the absence of doxicycline (the transgene is expressed), overall tail morphology is indistinguishable from controls
Genotype	Allelic Composition	Genetic Background
tg2	Eda^Ta-6J/Y Tg(MMTVtTA)1Mam/0 Tg(tetO-Eda*A1)1Dsch/0	involves: C57BL/6J
growth/size decreased body weight (J:86628) at 5 months of age, weight is about 80% of wild-type in the absence of doxicycline skin/coat/nails abnormal coat/ hair morphology (J:86628) in the presence of doxicycline throughout embryonic development to suppress Eda-A1 transgene expression, males are identical to Eda^Ta-6J males, showing lack of tail hair, guard hair and zigzag hair; mice continue to show this phenotype even after withdrawal of doxicycline from the food at 6 months of age disheveled coat (J:86628) in the absence of doxicycline, mutants have somewhat scruffy disordered coat hair compared to wild-type and Eda^Ta-6J males abnormal hair growth (J:86628) in the absence of doxicycline, tail hairs are reduced in number however, the hair behind the ears that is missing in Eda^Ta-6J males is fully restored when the transgene is expressed (absence of doxicycline) short hair (J:86628) in the absence of doxicycline, tail hairs are reduced in length abnormal hair types (J:86628) in the absence of doxicycline, the number of medium hairs is increased to a similar extent as in Eda^Ta-6J males however, total hair numbers are unchanged absent zigzag hairs (J:86628) zigzag hairs are not restored by transgene expression (absence of doxicycline) however, the number of guard hairs is restored to wild-type levels abnormal skin morphology (J:86628) tail ridges on skin surfaces are absent in the absence of doxicycline endocrine/exocrine glands abnormal gland morphology (J:86628) in the presence of doxicycline throughout embryonic development to suppress Eda-A1 transgene expression, males are identical to Eda^Ta-6J males, showing lack of sweat glands; mice continue to show this phenotype even after withdrawal of doxicycline from the food at 6 months of age abnormal sebaceous gland morphology (J:99510) transgene expression results in hypertrophy of sebaceous glands associated with hair follicles of the eyelids absent meibomian glands (J:99510, J:86628) meibomian glands are not restored by transgene expression (J:86628) however, Eda transgene expression does restore the sweat glands that are absent in Eda^Ta-6J males (J:86628) abnormal preputial gland morphology (J:86628) preputial gland is not restored by transgene expression enlarged sebaceous gland (J:86628) in the absence of doxicycline, sebaceous glands are 200-300% larger than in wild-type or Eda^Ta-6J males; size of individual sebocytes is the same but the numbers are increased, with excessive production of sebum hyperplasia is seen in both glands associated with each de novo guard follicle and also in the single sebaceous gland associated with each medium follicle addition of doxicycline at 3 months of age reverses the hyperplasia of sebaceous glands within 3 months vision/eye absent meibomian glands (J:99510, J:86628) meibomian glands are not restored by transgene expression (J:86628) however, Eda transgene expression does restore the sweat glands that are absent in Eda^Ta-6J males (J:86628) immune system immune system phenotype (J:99510) transgene expression rescues the susceptibility to corneal inflammation seen in Eda^Ta-6J males reproductive system abnormal preputial gland morphology (J:86628) preputial gland is not restored by transgene expression renal/urinary system abnormal preputial gland morphology (J:86628) preputial gland is not restored by transgene expression

References

Original:	J:86628 Cui CY et al., "Inducible mEDA-A1 transgene mediates sebaceous gland hyperplasia and differential formation of two types of mouse hair follicles." Hum Mol Genet 2003 Nov 15;12(22):2931-40
All:	3 reference(s)

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