Tg(GFAP-tTA)110Pop Transgene Detail MGI Mouse (MGI:3702613)

Tg(GFAP-tTA)110Pop
Transgene Detail

Nomenclature

Symbol:	Tg(GFAP-tTA)110Pop
Name:	transgene insertion 110, Brian Popko
MGI ID:	MGI:3702613
Synonyms:	GFAP-tTA, GFAP-tTA line 110, GFAP/tTA
Transgene:	Tg(GFAP-tTA)110Pop Location: unknown

Transgene
origin

Strain of Origin:

(C57BL/6 x DBA/2)F1

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	Expression of the tetracycline transactivator protein is under control of the promoter of the human glial fibrillary acidic protein gene. Five lines (67, 78, 96, 98 and 110) were established. (J:94092)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2	tg3
behavior/neurological			√	√
tremors			√	√
tonic seizures			√
ataxia				√
		tg1	tg2	tg3
growth/size			√
postnatal growth retardation			√
		tg1	tg2	tg3
lethality/postnatal		√
postnatal lethality		√
		tg1	tg2	tg3
life span/aging			√	N
life span/aging phenotype				N
premature death			√
		tg1	tg2	tg3
nervous system			√
tonic seizures			√
abnormal oligodendrocyte morphology			√
abnormal spinal cord morphology			√
abnormal spinal cord white matter morphology			√
abnormal myelination			√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Eif2ak3^tm1Dron/Eif2ak3^tm1Dron Tg(GFAP-tTA)110Pop/0 Tg(tetO-Ifng)184Pop/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2 * Swiss Webster
lethality/postnatal postnatal lethality (J:99655) pups die around P12 regardless whether dams received doxycycline to the end of gestation or until E14
Genotype	Allelic Composition	Genetic Background
tg2	Eif2ak3^tm1Dron/Eif2ak3⁺ Tg(GFAP-tTA)110Pop/0 Tg(tetO-Ifng)184Pop/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2 * Swiss Webster
life span/aging premature death (J:99655) greater than 90% of animals die by postnatal day 27 growth/size postnatal growth retardation (J:99655) pups are significantly smaller than controls littermates when doxycycline treatment is stopped on E14 nervous system tonic seizures (J:99655) >60% of animals display tonic seizures abnormal oligodendrocyte morphology (J:99655) when doxycycline treatment is stopped at E14, very few oligodendrocytes are detected in corpus callosum and cerebellum at P14 if mice are treated with doxycycline until they reach maturity (4 weeks) and then are released from doxycycline, oligodendrocyte survival is unaffected abnormal spinal cord morphology (J:99655) in cervical spinal cord at P14, number of apoptotic oligodendrocytes is significantly greater than in controls when doxycycline treatment is stopped at E14 abnormal spinal cord white matter morphology (J:99655) >80% of axons are unmyelinated compared to 30% of spinal cord axons in double mutants on a wild-type Eif2ak3 background when doxycycline treatment is stopped at E14; in mice continuously receiving doxycycline treatment, <10% of spinal cord axons are unmyelinated if mice are treated with doxycycline until they reach maturity (4 weeks) and then are released from doxycycline, normal myelin is observed in the central nervous system abnormal myelination (J:99655) level of myelin in CNS is significantly reduced at P14 compared to double transgenic mice with wild-type Eif2ak3 behavior/neurological tremors (J:99655) severe tremors are observed tonic seizures (J:99655) >60% of animals display tonic seizures
Genotype	Allelic Composition	Genetic Background
tg3	Tg(GFAP-tTA)110Pop/0 Tg(tetO-Ifng)184Pop/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2 * Swiss Webster
life span/aging life span-post-weaning/aging (J:99655) when doxycycline treatment is stopped at E14, double transgenic offspring exhibit good survival behavior/neurological tremors (J:99655) pups exhibit minor tremor ataxia (J:99655) pups exhibit minor ataxia

References

Original:	J:94092 Lin W et al., "Interferon-gamma induced medulloblastoma in the developing cerebellum." J Neurosci 2004 Nov 10;24(45):10074-83
All:	3 reference(s)

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