Trp53<tm5Tyj> Targeted Allele Detail MGI Mouse (MGI:3702077)

Trp53^tm5Tyj
Targeted Allele Detail

Nomenclature

Symbol:	Trp53^tm5Tyj
Name:	transformation related protein 53; targeted mutation 5, Tyler Jacks
MGI ID:	MGI:3702077
Synonyms:	p53-LSL, p53^LSL
Gene:	Trp53 Location: Chr11:69393861-69405375 bp, + strand Genetic Position: Chr11, 39.0 cM, cytoband B2-C

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:118233
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Mutation
description

Allele Type:	Targeted (Floxed/Frt)
Mutation:	Insertion
	A lox-stop-lox cassette is integrated into intron 1 disrupting expression of the gene. (J:118233)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Trp53 Mutation:	125 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	cn2	cn3	cx4
cellular		√	N		√
cellular phenotype			N
abnormal chromosome number		√
abnormal cell cycle		√
abnormal cell death		√
decreased cell proliferation					√
		hm1	cn2	cn3	cx4
embryogenesis					√
embryonic growth arrest					√
		hm1	cn2	cn3	cx4
lethality/prenatal-perinatal					√
embryonic lethality before implantation					√
		hm1	cn2	cn3	cx4
tumorigenesis				√
altered tumor susceptibility				√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Trp53^tm5Tyj/Trp53^tm5Tyj	involves: 129S4/SvJae
cellular abnormal chromosome number (J:118233) marked genetic instability similar to that seen in Trp53^tm1Tyj homozygous cells abnormal cell cycle (J:96433) similar to Trp53 null cells, the percentage of cells in S phase is increased compared to wild-type cells abnormal cell death (J:118233, J:96433) lack cell-culture-induced senescence (J:118233) in the absence of cre, MEFs are refractory to stress-induced cell death (J:96433)
Genotype	Allelic Composition	Genetic Background
cn2	Trp53^tm5Tyj/Trp53^tm5Tyj	involves: 129S4/SvJae
cellular cellular phenotype (J:96433) infection with adenoviral cre restores normal DNA damage-induce G1 arrest response and stress-induced apoptotic responses
Genotype	Allelic Composition	Genetic Background
cn3	Gt(ROSA)26Sor^{tm1(cre/ESR1)Tyj}/? Trp53^tm5Tyj/Trp53^tm5Tyj	involves: 129S4/SvJae * C57BL/6
tumorigenesis altered tumor susceptibility (J:118233) mice with radiation-induced tumors that are subsequently treated with tamoxifen show tumor regression (7 of 10) or cessation of tumor progression (2 of 10)
Genotype	Allelic Composition	Genetic Background
cx4	Gnl3^{Gt(W223E05)Wrst}/Gnl3^{Gt(W223E05)Wrst} Trp53^tm1Tyj/Trp53^tm5Tyj	involves: 129S2/SvPas * 129S4/SvJae
lethality/prenatal-perinatal embryonic lethality before implantation (J:118144) loss of Trp53 expression does not alter or delay the lethality embryogenesis embryonic growth arrest (J:118144) loss of Trp53 expression does not alter or delay the overall phenotype cellular decreased cell proliferation (J:118144) loss of Trp53 expression does not alter or delay the impairment of cell proliferation

References

Original:	J:118233 Ventura A et al., "Restoration of p53 function leads to tumour regression in vivo." Nature 2007 Feb 8;445(7128):661-5
All:	5 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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