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| Nomenclature |
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Symbol:
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Mecp2tm2Bird
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Name:
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methyl CpG binding protein 2;
targeted mutation 2, Adrian Bird
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MGI ID: |
MGI:3700191 |
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Synonyms: |
Mecp2lox-stop |
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Gene:
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Mecp2
Location:
ChrX:71271931-71331029 bp, - strand
Genetic Position: ChrX,
29.6 cM
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Mutation
origin |
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Germline Transmission:
| Earliest citation of germline transmission:
J:118365
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Parent Cell Line:
| E14TG2a (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: |
Targeted (Floxed/Frt) |
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Mutation: |
Insertion |
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Cre recombinase-reversible gene inactivation was accomplished by insertion into intron 2 of a 3.1-kb DNA fragment containing a loxP-flanked "neostop" cassette, which comprises a neomycin resistance cassette followed by a transcriptional/translational "stop" cassette composed of 550 bp of 3' sequence from the Saccharomyces cerevisiae His3 gene, an SV40 polyadenylation sequence, and a synthetic sequence containing a false translation initiation codon (ATG) immediately followed by a consensus splice donor sequence. Despite the production of a read-through transcript, no protein product was detected by western blot or immunofluorescence analysis of brains of mutant mice. (J:75205, J:111323, J:118365) |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
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Key:
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| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
 |
expected model not found |
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Phenotypic
data by
genotype
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Phenotypic Data by Genotype
(show or
hide all phenotypic details)
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Disease
models
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| References |
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Original: |
J:118365
Guy J et al.,
"Reversal of neurological defects in a mouse model of Rett syndrome."
Science 2007 Feb 23;315(5815):1143-7
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All: |
3 reference(s)
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