|
|
| Nomenclature |
|
Symbol:
|
Tg(PF4-tTA/VP16)42Kra
|
|
Name:
|
transgene insertion 42, Katya Ravid
|
|
MGI ID: |
MGI:3656262 |
|
Synonyms: |
PF4-tTA-VP16 |
|
Transgene:
|
Tg(PF4-tTA/VP16)42Kra
Location:
unknown
|
|
Transgene
origin |
|
Transgene
description |
|
Transgene
Type: |
Transgenic (random, expressed) |
|
Mutation: |
Insertion |
| |
The rat platelet factor 4 (RP4) promoter was cloned into the tTA-containing plasmid, replacing the existing CMV promoter. The promoter will drive expression of the transactivator tTA fused to the viral coactivator VP16. Expression is repressed with administration of doxycycline and is induced by removal of doxycycline from the diet of the mice.The transgene also contains 1.7 kb of the 3'end of the human beta-globin gene including the 3' intron and polyadenylation signal to permit stable RNA production in mice. A stop codon in the transgene prevents translation of beta-globin into the protein. (J:104239) |
|
| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
|
Phenotype
summary
|
|
Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
|
Key:
|
| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
 |
expected model not found |
|
|
|
|
|
Phenotypic
data by
genotype
|
|
Phenotypic Data by Genotype
(show or
hide all phenotypic details)
|
|
|
|
| References |
|
Original: |
J:104239
Nguyen HG et al.,
"Conditional overexpression of transgenes in megakaryocytes and platelets in vivo."
Blood 2005 Sep 1;106(5):1559-64
|
|
All: |
1 reference(s)
|
|
