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| Nomenclature |
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Symbol:
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Tg(TettTALuc)1Dgs
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Name:
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transgene insertion 1, David Schatz
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MGI ID: |
MGI:3525014 |
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Transgene:
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Tg(TettTALuc)1Dgs
Location:
unknown
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Transgene
description |
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Transgene
Type: |
Transgenic (Reporter) |
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Mutation: |
Insertion |
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Transgenic mice were produced by co-microinjection of modified tetracycline-controlled transactivator (tTA) and luciferase genes placed under the control of tetracycline-responsive promoter elements (TREs). (J:95091) |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Notes |
This line was generated from founder #17. Homozygous transgenic mice are viable and fertile. Expression of tTA in transgenic mice is both inducible and autoregulatory; expression of the transgenes are suppressed in the presence of tetracycline and highly expressed in its absence. Levels of induced transgene expression in these mice are higher than those achieved in mice carrying a transgene that utilizes a constitutive tTA system. Luciferase (in the absence of tetracycline) is expressed in spleen, thymus, lung, liver, kidney, heart, cerebrum, cerebellum, lymph nodes, and testes. High activity is found in thymus and lung; low expression is found in liver and kidney. Induction ranges from 2-fold in testes to 150-fold in thymus.
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| References |
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Original: |
J:95091
Shockett P et al.,
"A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice."
Proc Natl Acad Sci U S A 1995 Jul 3;92(14):6522-6
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All: |
2 reference(s)
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