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Tg(APPSw)40Btla
Transgene Detail

Nomenclature
Symbol: Tg(APPSw)40Btla
Name: transgene insertion 40, Bruce Lamb
MGI ID: MGI:3510594
Synonyms: APPK670/M671, APPK670N/M671L, R1.40, R1.40-YAC
Transgene: Tg(APPSw)40Btla   Location: unknown     Genetic Position: Chr13, Syntenic
Transgene
origin
Germline Transmission: Earliest citation of germline transmission: J:42613
Parent Cell Line: R1 (ES Cell)
Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl+
Transgene
description
Transgene Type: Transgenic (random, expressed)
Mutation: Insertion
  The transgenic insertion comprises four to eight copies of a 650-kb YAC containing the entire 400-kb human amyloid precursor protein gene with the Swiss familial Alzheimer disease (FAD) mutation and approximately 250 kb of flanking human DNA. The double mutation (K670N/M671N) comprises a G-to-A transition converting the lysine codon at position 670 to an asparagine codon and an A-to-C transversion replacing the methionine at amino acid 671 with leucine. RT-PCR and western blot analysis revealed that hemizygous transgenic mice express all major human APP mRNA and protein isoforms in brain, in parallel with the corresponding mouse versions, at approximately three times the levels of the latter. Total brain levels of the Alzheimer disease- associated 42-amino acid amyloid-beta peptide detected by ELISA are 7-8-fold and 15-20-fold higher, respectively, in mice hemizygous and homozygous for the mutant gene than in hemizygotes for the wildtype human gene. Levels of a cell-associated, beta-secretase-generated 13.5 kDa C-terminal peptide containing the amyloid beta domain and of soluble amyloid beta peptides are significantly elevated in brains of mice with the mutant, versus wildtype, transgene, while alpha-secretase products are diminished. (J:42613, J:58050, J:73622)
Find Mice (IMSR) Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation: Mouse Strains: 5 strains available      Cell Lines: 0 lines available
Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

      Key:  
hm homozygous ht heterozygous
cn conditional genotype  cx complex: > 1 genome feature
tg involves transgenes ot other: hemizygous, indeterminate,...
N normal phenotype expected model not found
Affected SystemsGenotypes:
 
tg1
 
tg2
 
tg3
 
tg4
 
tg5
 
tg6
 
tg7
 
tg8
 
tg9
 
tg10
 
tg11
 
tg12
 
tg13
 
tg14
 
tg15
  
nervous system          		√N√√√√√√√√√√√√√
  
other phenotype          		√ √√√√√√√√√√√√√
 
  
Disease Models          		√  √           
Phenotypic
data by
genotype
Phenotypic Data by Genotype

(show or hide all phenotypic details)

GenotypeAllelic CompositionGenetic Background
  
 tg1   Disease Model  		 Tg(APPSw)40Btla/Tg(APPSw)40Btla B6.129-Tg(APPSw)40Btla
  
 tg2   		 Tg(APPSw)40Btla/Tg(APPSw)40Btla D2.129-Tg(APPSw)40Btla
  
 tg3   		 Tg(APPSw)40Btla/Tg(APPSw)40Btla involves: 129S1/Sv * 129X1/SvJ
  
 tg4   Disease Model  		 Tg(APPSw)40Btla/0 involves: 129S1/Sv * 129X1/SvJ
  
 tg5   		 Tg(APPSw)40Btla/0
Tg(PSEN1H163R)G9Btla/0		 involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
  
 tg6   		 Tg(APPSw)40Btla/Tg(APPSw)40Btla
Tg(PSEN1H163R)G9Btla/0		 involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ
  
 tg7   		 Brabq1DBA/2J/Brabq1DBA/2J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg8   		 Brabq1C57BL/6J/Brabq1DBA/2J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg9   		 Brabq1C57BL/6J/Brabq1C57BL/6J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg10   		 Brabq2DBA/2J/Brabq2DBA/2J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg11   		 Brabq2C57BL/6J/Brabq2DBA/2J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg12   		 Brabq2C57BL/6J/Brabq2C57BL/6J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg13   		 Brabq3DBA/2J/Brabq3DBA/2J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg14   		 Brabq3C57BL/6J/Brabq3DBA/2J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
  
 tg15   		 Brabq3C57BL/6J/Brabq3C57BL/6J
Tg(APPSw)40Btla/0		 involves: C57BL/6J * DBA/2J
Disease
models
Mouse Models
of Human Disease
NoteGenotypeRef(s)
 
Allelic Composition
Genetic Background
Models involving transgenes or other mutation types.1
Alzheimer Disease; AD
OMIM ID: 104300		  
 
tg1		Tg(APPSw)40Btla/Tg(APPSw)40BtlaB6.129-Tg(APPSw)40BtlaJ:86629
 
 
tg4		Tg(APPSw)40Btla/0involves: 129S1/Sv * 129X1/SvJJ:42613
1Models involving transgenes or other mutation types may also appear in other sections of the table.
References
Original: J:42613 Lamb BT et al., "Altered metabolism of familial Alzheimer's disease-linked amyloid precursor protein variants in yeast artificial chromosome transgenic mice." Hum Mol Genet 1997 Sep;6(9):1535-41
All: 9 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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11/20/2009
MGI_4.31
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