Tg(MMTVTGFA)29Rjc Transgene Detail MGI Mouse (MGI:3040871)

Tg(MMTVTGFA)29Rjc
Transgene Detail

Nomenclature

Symbol:	Tg(MMTVTGFA)29Rjc
Name:	transgene insertion 29, Robert J Coffey
MGI ID:	MGI:3040871
Transgene:	Tg(MMTVTGFA)29Rjc Location: unknown

Transgene
origin

Strain of Origin:

(C57BL/6 x DBA/2)F2

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	This transgene contains the mouse mammary tumor virus LTR and the normal allele of the human transforming growth factor alpha gene. The vector also contains exons 2 and 3 of the rabbit beta-globin gene. Lines 254, 257, 64 and 29 were produced. (J:28453)

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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2	tg3	tg4
behavior/neurological		√			√
abnormal nursing		√			√
		tg1	tg2	tg3	tg4
endocrine/exocrine glands		√		√	√
endocrine/exocrine glands phenotype		N
abnormal mammary gland morphology		√
abnormal mammary gland growth during pregnancy		√
mammary gland hyperplasia		√			√
abnormal mammary gland duct morphology					√
mammary gland duct hyperplasia		√
abnormal mammary gland alveolus morphology					√
mammary gland alveolar hyperplasia		√		√
		tg1	tg2	tg3	tg4
reproductive system		√		√	√
abnormal mammary gland morphology		√
abnormal mammary gland growth during pregnancy		√
mammary gland hyperplasia		√			√
abnormal mammary gland duct morphology					√
mammary gland duct hyperplasia		√
abnormal mammary gland alveolus morphology					√
mammary gland alveolar hyperplasia		√		√
		tg1	tg2	tg3	tg4
tumorigenesis		√	√	N	√
tumorigenesis phenotype				N
squamous cell carcinoma		√
mammary gland tumor		√			√
mammary adenocarcinoma		√	√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Tg(MMTVTGFA)29Rjc/0	involves: C57BL/6 * DBA/2
endocrine/exocrine glands endocrine/exocrine gland phenotype (J:28453) male mice exhibit normal mammary and salivary gland abnormal mammary gland morphology (J:28453, J:72085, J:35344) after formation of tumors at 3 months, glands are small with minimal secretion in the lumen and the stroma around the glands is abundant but mitosis and dysplasia of the epithelial or stromal cells is not observed (J:28453) stroma between the mammary glands is hyperplastic (J:28453) at 5 months two females develop bilateral swollen, lumpy thoracic mammary glands with abundant alveolar and ductal proliferation containing abundant secretions (J:28453) at 5 months of age, female mice exhibit epithelial and stromal proliferation (J:28453) the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453) at 4 months of age, female mice develop numerous lumps within thoracic and inguinal areas of the mammary glands with large cystic spaces and abundant secretion within the lumen along with solid hyperplasias (J:72085) female mice exhibit abnormal ductal trees (J:35344) female mice exhibit cystically dilated alveoli (J:35344) abnormal mammary gland growth during pregnancy (J:28453, J:72085) during pregnancy, female mice exhibit increased stromal cell proliferation, more prominent nuclei of epithelial cells, and production of more secretion than in wild-type mice (J:28453) after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453) during pregnancy, female mice exhibit abnormal mammary gland hyperplasia with cysts that are more prominent and secretions that are more inspissated than in wild-type mice (J:72085) mammary gland duct hyperplasia (J:28453) at 5 months of age mammary gland alveolar hyperplasia (J:28453, J:72085) at day 90 (J:72085) mammary gland hyperplasia (J:71696, J:35344) female mice exhibit lobular development similar to a lactating wild-type mice except that the alveoli are irregular and dilated with interstitial stroma edema and a modest increase in leukocyte infiltration (J:35344) virgin females exhibit papillary hyperplasia (J:35344) however, nulliparous mice exhibit minimal lobular development and no stromal response (J:35344) tumorigenesis squamous cell carcinoma (J:72080) following treatment with 0.5 mg DMBA at 21 or 56 days after birth, predominantly mammary adenocarcinomas form with occasional squamous differentiation mammary gland tumor (J:28453, J:72085, J:71696, J:35344, J:72080) after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453) tumors have fibrous encapsulation with no evidence of local invasion and are composed of numerous well-formed alveoli with rare mitotic figures (J:28453) adenomas develop in multiparous female mice (J:72085) at 17 months, 53% of female mice exhibit solid tumors (J:71696) in 6% of female mice at day 250 (J:35344) following treatment with 0.5 mg DMBA at 56 days after birth, 50% of mice develop mammary gland tumors at day 180 and 65% at day 250 compared to only 6% of similarly treated wild-type mice at day 250 (J:72080) following treatment with 0.5 mg DMBA at 56 days after birth, mice develop an average of 3.6 tumors per mouse with 60% of tumors arising in the thoracic mammary gland and 31% in the inguinal mammary gland (J:72080) following treatment with 0.5 mg DMBA at 21 days after birth, 50% of mice develop mammary tumors within 250 days at a frequency of 2.4 tumors per mouse compared to no tumor development in similarly treated wild-type mice (J:72080) tumor onset is delayed when mice are treated treatment with 0.5 mg DMBA at 21 days after birth compared to at 56 days after birth (J:72080) mammary adenocarcinoma (J:28453, J:72085, J:71696, J:72080) the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453) solid hyperplasias develop into adenocarcinomas (J:72085) in 40% of female mice (J:71696) following treatment with 0.5 mg DMBA at 21 or 56 days after birth, predominantly mammary adenocarcinomas form with occasional squamous differentiation (J:72080) behavior/neurological abnormal nursing (J:28453, J:72085) females cannot suckle her young (J:28453) females cannot nurse her young (J:72085) reproductive system abnormal mammary gland morphology (J:28453, J:72085, J:35344) after formation of tumors at 3 months, glands are small with minimal secretion in the lumen and the stroma around the glands is abundant but mitosis and dysplasia of the epithelial or stromal cells is not observed (J:28453) stroma between the mammary glands is hyperplastic (J:28453) at 5 months two females develop bilateral swollen, lumpy thoracic mammary glands with abundant alveolar and ductal proliferation containing abundant secretions (J:28453) at 5 months of age, female mice exhibit epithelial and stromal proliferation (J:28453) the female founder mouse developed a mass during pregnancy that receded followed by development of masses in the inguinal fat pad and soft tissues of the upper thorax consisting of adenocarcinoma without metastasis (J:28453) at 4 months of age, female mice develop numerous lumps within thoracic and inguinal areas of the mammary glands with large cystic spaces and abundant secretion within the lumen along with solid hyperplasias (J:72085) female mice exhibit abnormal ductal trees (J:35344) female mice exhibit cystically dilated alveoli (J:35344) abnormal mammary gland growth during pregnancy (J:28453, J:72085) during pregnancy, female mice exhibit increased stromal cell proliferation, more prominent nuclei of epithelial cells, and production of more secretion than in wild-type mice (J:28453) after 3 month, female mice that have been pregnant develop an inguineal mammary fat pad that develops into a circumscribed, firm tumor (J:28453) during pregnancy, female mice exhibit abnormal mammary gland hyperplasia with cysts that are more prominent and secretions that are more inspissated than in wild-type mice (J:72085) mammary gland duct hyperplasia (J:28453) at 5 months of age mammary gland alveolar hyperplasia (J:28453, J:72085) at day 90 (J:72085) mammary gland hyperplasia (J:71696, J:35344) female mice exhibit lobular development similar to a lactating wild-type mice except that the alveoli are irregular and dilated with interstitial stroma edema and a modest increase in leukocyte infiltration (J:35344) virgin females exhibit papillary hyperplasia (J:35344) however, nulliparous mice exhibit minimal lobular development and no stromal response (J:35344)
Genotype	Allelic Composition	Genetic Background
tg2	Tg(MMTV-TGFBR2)7Hlm/? Tg(MMTVTGFA)29Rjc/?	involves: C57BL/6 * DBA
tumorigenesis mammary adenocarcinoma (J:85799) 17 of 19 mice develop mammary tumors with 5 mice having tumors with invasive characteristics the number of invasive tumors is significantly less than mice carrying just the Tg(MMTVTGFA)29Rjc transgene 15 of the 17 mice with tumors had tumors in multiple mammary glands tumor development occurs with a median latency of 9 months
Genotype	Allelic Composition	Genetic Background
tg3	Tg(MMTVTGFA)29Rjc/0 Tg(MMTVTGFB1)46Hlm/0	involves: C57BL/6 * DBA/2
endocrine/exocrine glands mammary gland alveolar hyperplasia (J:71696) widely scattered hyperplastic alveolar outgrowths are present tumorigenesis tumorigenesis (J:71696) unlike mice overexpressing TGFA, mice do not exhibit mammary tumors reproductive system mammary gland alveolar hyperplasia (J:71696) widely scattered hyperplastic alveolar outgrowths are present
Genotype	Allelic Composition	Genetic Background
tg4	Tg(MMTVneu)202Mul/0 Tg(MMTVTGFA)29Rjc/0	involves: C57BL/6 * DBA/2 * FVB/N
endocrine/exocrine glands abnormal mammary gland duct morphology (J:35344) female mice exhibit abnormal ductal trees abnormal mammary gland alveolus morphology (J:35344) alveoli exhibit denser cell lining in the walls compared to Tg(MMTVTGFA)29Rjc expressing mice that exhibit cystically dilated alveoli mammary gland hyperplasia (J:35344) nulliparous female mice exhibit epithelial hyperplasia and dysplasia along with stromal inflammation tumorigenesis mammary gland tumor (J:35344) 50% of mice exhibit mammary gland tumors at day 175 and 95% at day 250 compared to 6% of Tg(MMTVTGFA)29Rjc expressing mice and 35% of Tg(MMTVneu)202Mul expressing mice at day 250 mice exhibit tumors that are nodular or tubular but not mixed behavior/neurological abnormal nursing (J:35344) females cannot nurse her young reproductive system abnormal mammary gland duct morphology (J:35344) female mice exhibit abnormal ductal trees abnormal mammary gland alveolus morphology (J:35344) alveoli exhibit denser cell lining in the walls compared to Tg(MMTVTGFA)29Rjc expressing mice that exhibit cystically dilated alveoli mammary gland hyperplasia (J:35344) nulliparous female mice exhibit epithelial hyperplasia and dysplasia along with stromal inflammation

References

Original:	J:28453 Matsui Y et al., "Development of mammary hyperplasia and neoplasia in MMTV-TGF alpha transgenic mice." Cell 1990 Jun 15;61(6):1147-55
All:	13 reference(s)

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