Tg(MMTVTGFB1)46Hlm Transgene Detail MGI Mouse (MGI:3040376)

Tg(MMTVTGFB1)46Hlm
Transgene Detail

Nomenclature

Symbol:	Tg(MMTVTGFB1)46Hlm
Name:	transgene insertion 46, Harold L Moses
MGI ID:	MGI:3040376
Transgene:	Tg(MMTVTGFB1)46Hlm Location: unknown

Transgene
origin

Strain of Origin:

(C57BL/6 x DBA/2)F2

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	This transgene contains the mouse mammary tumor virus promoter and a modified allele of simian transforming growth factor, beta 1. In this modified allele cystein codons at positions 223 and 225 were changed to serine codons (this generates an active form of TGFB1). The construct also contained exons 2 and 3 of the rabbit beta-globin gene. (J:72128)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 2 strains available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2	tg3
endocrine/exocrine glands		√	√	√
endocrine/exocrine glands phenotype		N
abnormal mammary gland morphology		√
abnormal branching of the mammary ductal tree		√
abnormal mammary gland epithelium morphology		√
abnormal mammary gland lobule morphology		√
mammary gland alveolar hyperplasia				√
mammary gland hyperplasia			√
		tg1	tg2	tg3
reproductive system		√	√	√
abnormal mammary gland morphology		√
abnormal branching of the mammary ductal tree		√
abnormal mammary gland epithelium morphology		√
abnormal mammary gland lobule morphology		√
mammary gland alveolar hyperplasia				√
mammary gland hyperplasia			√
		tg1	tg2	tg3
tumorigenesis		√		N
tumorigenesis phenotype		N		N
decreased incidence of chemically-induced tumors		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
tg1	Tg(MMTVTGFB1)46Hlm/0	involves: C57BL/6 * DBA/2
endocrine/exocrine glands endocrine/exocrine gland phenotype (J:72128) despite abnormal morphology, mice are still able to produce milk and normally nurse their young abnormal mammary gland morphology (J:72128) abnormal branching of the mammary ductal tree (J:72128, J:71696) mammary duct tree branching development is suppressed due to decreased proliferation of mammary epithelial cells (J:72128) at week 13, lateral branching is virtually absent (J:72128) multiparous mice exhibit reduced ductal branching compared to wild-type mice (J:71696) abnormal mammary gland epithelium morphology (J:72128) during lactation ductal and alveolar epithelial cells appear more flattened and somewhat atrophic compared to in wild-type mice abnormal mammary gland lobule morphology (J:72128) pregnant and lactating glands exhibit fewer lobules than in wild-type mice tumorigenesis tumorigenesis (J:72128) unlike with overexpression of TGFA, mice do not exhibit mammary tumors decreased incidence of chemically-induced tumors (J:71696) mice exhibit fewer DMBA-induced tumors than similarly treated wild-type mice reproductive system abnormal mammary gland morphology (J:72128) abnormal branching of the mammary ductal tree (J:72128, J:71696) mammary duct tree branching development is suppressed due to decreased proliferation of mammary epithelial cells (J:72128) at week 13, lateral branching is virtually absent (J:72128) multiparous mice exhibit reduced ductal branching compared to wild-type mice (J:71696) abnormal mammary gland epithelium morphology (J:72128) during lactation ductal and alveolar epithelial cells appear more flattened and somewhat atrophic compared to in wild-type mice abnormal mammary gland lobule morphology (J:72128) pregnant and lactating glands exhibit fewer lobules than in wild-type mice
Genotype	Allelic Composition	Genetic Background
tg2	Rb1^tm1Fad/Rb1^tm1Fad Tg(MMTVTGFB1)46Hlm/?	involves: 129 * C57BL/6 * DBA/2
endocrine/exocrine glands mammary gland hyperplasia (J:151417) mammary glands have hyperplastic growth throughout development hyperplasia was characterized by increased luminal epithelial cell layers, as well as invagination of the epithelium into the lumen of the duct this genotype demonstrates that mammary hyperplasia associated with Rb1^tm1Fad homozygotes still occurs in the presence of high levels of TGF-beta1 reproductive system mammary gland hyperplasia (J:151417) mammary glands have hyperplastic growth throughout development hyperplasia was characterized by increased luminal epithelial cell layers, as well as invagination of the epithelium into the lumen of the duct this genotype demonstrates that mammary hyperplasia associated with Rb1^tm1Fad homozygotes still occurs in the presence of high levels of TGF-beta1
Genotype	Allelic Composition	Genetic Background
tg3	Tg(MMTVTGFA)29Rjc/0 Tg(MMTVTGFB1)46Hlm/0	involves: C57BL/6 * DBA/2
endocrine/exocrine glands mammary gland alveolar hyperplasia (J:71696) widely scattered hyperplastic alveolar outgrowths are present tumorigenesis tumorigenesis (J:71696) unlike mice overexpressing TGFA, mice do not exhibit mammary tumors reproductive system mammary gland alveolar hyperplasia (J:71696) widely scattered hyperplastic alveolar outgrowths are present

References

Original:	J:72128 Pierce DF Jr et al., "Inhibition of mammary duct development but not alveolar outgrowth during pregnancy in transgenic mice expressing active TGF-beta 1." Genes Dev 1993 Dec;7(12A):2308-17
All:	4 reference(s)

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