Tg(APP)8.9Btla Transgene Detail MGI Mouse (MGI:2447591)

Tg(APP)8.9Btla
Transgene Detail

Nomenclature

Symbol:	Tg(APP)8.9Btla
Name:	transgene insertion 8.9, Bruce Lamb
MGI ID:	MGI:2447591
Synonyms:	APPYAC, hABetaPP, Py8.9, Tg(APP)C9.24Jgh
Transgene:	Tg(APP)8.9Btla Location: unknown

Transgene
origin

Germline Transmission:	Earliest citation of germline transmission: J:80947
Parent Cell Line:	D3 (ES Cell)
Strain of Origin:	129S2/SvPas

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	A 650 kb YAC containing the entire human APP gene and 350 kb of flanking sequence was randomly integerated into ES cells. Southern blot analysis confirmed that construct rearrangement did not occur and that the animals carried a single copy of the intact transgene. Quantitative RT-PCR analysis of transgenic and wild-type mice revealed that the levels of transgenic human alternate transcripts corresponded with those of the endogenous alternative transcripts. The human APP promoter drives expression in the brain, heart, kidney and testes. (J:80947)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2	tg3
behavior/neurological		√	√	√
decreased anxiety-related response		√
abnormal locomotor activation		√	√
increased anxiety-related response			√
abnormal learning/ memory			√	√
increased exploration in new environment		√	√
abnormal short term object recognition memory		√	√	√
abnormal spatial learning		√	√	√
		tg1	tg2	tg3
nervous system			√	√
reduced long term potentiation			√	√
abnormal excitatory postsynaptic potential				√
		tg1	tg2	tg3
other phenotype		N	√	√
other phenotype phenotype		N	N
amyloidosis			√	√
		tg1	tg2	tg3
vision/eye		√
vision/eye phenotype		N
abnormal lens morphology		√
abnormal lens fiber morphology		√

		tg1	tg2	tg3
Disease Models		√
Alzheimer Disease; AD		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype

Allelic Composition

Genetic Background

tg1 Disease Model

Tg(APP)8.9Btla/Tg(APP)8.9Btla

involves: 129S2/SvPas

behavior/neurologicaldecreased anxiety-related response (J:96742)

mice spend less time per entry into dark arms than controlsmice spend less time per entry into dark arms than controls

mice exit dark side of dark-light box more often than controls

increased exploration in new environment (J:96742)

exploratory behavior is increased for both familiar and novel objects

abnormal short term object recognition memory (J:96742)

at 5.5 months of age, mice do not appear to recall platform position from the previous day of training and show a lower learning rate over subsequent trials

impairment observed at 10 months of age

abnormal spatial learning (J:96742)

at 9 months, a delay of learning process in Morris water maze is observed compared to controls

abnormal locomotor activation (J:96742)

transgenic mice display more spontaneous locomotor activity than controls; activity is higher than in Tg(APP)8.9Btla or Tg(SOD1)51Yg mice

other phenotypeother phenotype (J:96742)

no Abeta peptides are detected in 5 month old mice

vision/eyevision/eye phenotype (J:80534)

little or no perturbation of the retinas structure or cornea is observed

abnormal lens morphology (J:80534)

numerous bodies with circular profiles are present in all lens sections examined

circles are present in lenses from mice >6 months of age; circles are 1-5 um in diameter

lenses from 6 and 16 month-old mice contain swollen cortical fiber cells and lens plaques

fiber cell nuclei are disorganized in the outer cortical region compared to wild-type

lens degeneration is variable in severity and onset; in some cases, one lens is more severely affected than the other side

abnormal lens fiber morphology (J:80534)

individual fiber cells are irregularly shaped and fiber cell arrays are misaligned

lens fiber cell membranes are morphologically abnormal

mice display age-related fiber cell degeneration and cortical plaque formation

Mouse Models of Human Disease	Note	Ref(s)
Models involving transgenes or other mutation types.¹
Alzheimer Disease; AD		J:96742

¹Models involving transgenes or other mutation types may also appear in other sections of the table.

tg2

Tg(APP)8.9Btla/Tg(APP)8.9Btla
Tg(SOD1)51Yg/Tg(SOD1)51Yg

involves: 129S2/SvPas * BALB/c * C57BL/6J * DBA/2

tg3

Tg(APP)8.9Btla/Tg(APP)8.9Btla
Tg(SOD1)69Yg/Tg(SOD1)69Yg

involves: 129S2/SvPas * BALB/c * C57BL/6J * DBA/2

Disease
models

Mouse Models of Human Disease	Note	Genotype			Ref(s)
			Allelic Composition	Genetic Background
Models involving transgenes or other mutation types.¹
Alzheimer Disease; AD OMIM ID: 104300		tg1	Tg(APP)8.9Btla/Tg(APP)8.9Btla	involves: 129S2/SvPas	J:96742

¹Models involving transgenes or other mutation types may also appear in other sections of the table.

References

Original:	J:80947 Lamb BT et al., "Introduction and expression of the 400 kilobase amyloid precursor protein gene in transgenic mice [corrected]" Nat Genet 1993 Sep;5(1):22-30
All:	7 reference(s)

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