Tg(MMTV-TGFBR2)7Hlm Transgene Detail MGI Mouse (MGI:2429406)

Tg(MMTV-TGFBR2)7Hlm
Transgene Detail

Nomenclature

Symbol:	Tg(MMTV-TGFBR2)7Hlm
Name:	transgene insertion 7, Harold L Moses
MGI ID:	MGI:2429406
Synonyms:	MMTV-DNIIR-7
Transgene:	Tg(MMTV-TGFBR2)7Hlm Location: unknown

Transgene
origin

Strain of Origin:

(C57BL/6 x DBA)F2

Transgene
description

Transgene Type:	Transgenic (random, expressed)
Mutation:	Insertion
	A human cDNA encoding a carboxy-terminally truncated TGFBR2 was adjoined to an MMTV regulatory region. While the encoded receptor contains the complete extracellular and transmembrane domains and is therefore capable of ligand binding and and interacting with type 1 receptors, it lacks the kinase domain. Expression was driven in mammary epithelial cells by the MMTV LTR promoter/enhancer. Transgenic expression was verified by Northern blot analysis. (J:46432)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	tg1	tg2	tg3
endocrine/exocrine glands		√	√
abnormal involution of the mammary gland		√
abnormal mammary gland growth during lactation		√
mammary gland alveolar hyperplasia		√	√
mammary gland hypoplasia		√
abnormal lactation		√	√
		tg1	tg2	tg3
reproductive system		√	√
abnormal involution of the mammary gland		√
abnormal mammary gland growth during lactation		√
mammary gland alveolar hyperplasia		√	√
mammary gland hypoplasia		√
abnormal lactation		√	√
		tg1	tg2	tg3
tumorigenesis		√		√
mammary adenocarcinoma		√		√

		tg1	tg2	tg3
Disease Models		√
Breast Cancer		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype

Allelic Composition

Genetic Background

tg1 Disease Model

Tg(MMTV-TGFBR2)7Hlm/Tg(MMTV-TGFBR2)7Hlm

involves: C57BL/6 * DBA

endocrine/exocrine glandsabnormal involution of the mammary gland (J:85799)

there is consistent retardation of involution observed in mice both 7 and 10 days after weaning compared to control mice

abnormal mammary gland growth during lactation (J:85799)

there is accelerated development and differentiation of mammary epithelial cells during early to mid-pregnancy but impaired development during the last stages of pregnancy

mammary gland alveolar hyperplasia (J:46432, J:85799)

alveolar hyperplasia is evident in non-pregnant females while no alveolar structures are observed in non-pregnant wild-type mice (J:46432)

the alveolar-like buds fill in the spaces between the ductal structure of the mammary gland (J:46432)

the morphology of the mammary gland resembles the morphology found in pregnant wild-type mice at early to mid-gestation (J:46432)

the hyperplasia observed is more severe than observed in mice hemizyguos for the transgene (J:46432)

mice 15 days into their first pregnancy have more prominent vacuoles in the alveoli than in control mice (J:85799)

the alveolar are also in a more differentiated state as indicated by the presence of milk proteins in the lumen (J:85799)

mammary gland hypoplasia (J:85799)

mice have fewer alveoli 1 day after the onset of lactation than do control mice

abnormal lactation (J:46432, J:85799)

the milk protein beta-Casein is detectable in the alveolar buds of non-pregnant females (J:46432)

the concentration and location of the beta-Casein is similar to what is found in 12.5-day pregnant females (J:46432)

mice are frequently unable to feed their pups (J:85799)

while alveolar hyperplasia with some milk production occurs early in pregnancy (15 days), the mammary glands fail to develop into fully functional organs as indicated by less extensive alveolar development and decreased milk production compared to non-transgenic controls (J:85799)

reproductive systemabnormal involution of the mammary gland (J:85799)

there is consistent retardation of involution observed in mice both 7 and 10 days after weaning compared to control mice

abnormal mammary gland growth during lactation (J:85799)

there is accelerated development and differentiation of mammary epithelial cells during early to mid-pregnancy but impaired development during the last stages of pregnancy

mammary gland alveolar hyperplasia (J:46432, J:85799)

alveolar hyperplasia is evident in non-pregnant females while no alveolar structures are observed in non-pregnant wild-type mice (J:46432)

the alveolar-like buds fill in the spaces between the ductal structure of the mammary gland (J:46432)

the morphology of the mammary gland resembles the morphology found in pregnant wild-type mice at early to mid-gestation (J:46432)

the hyperplasia observed is more severe than observed in mice hemizyguos for the transgene (J:46432)

mice 15 days into their first pregnancy have more prominent vacuoles in the alveoli than in control mice (J:85799)

the alveolar are also in a more differentiated state as indicated by the presence of milk proteins in the lumen (J:85799)

mammary gland hypoplasia (J:85799)

mice have fewer alveoli 1 day after the onset of lactation than do control mice

abnormal lactation (J:46432, J:85799)

the milk protein beta-Casein is detectable in the alveolar buds of non-pregnant females (J:46432)

the concentration and location of the beta-Casein is similar to what is found in 12.5-day pregnant females (J:46432)

mice are frequently unable to feed their pups (J:85799)

tumorigenesismammary adenocarcinoma (J:85799)

mice that have experienced two rounds of pregnancy have a frequency of spontaneous mammary carcinoma

47% of these females develop mammary tumors with a median latency of 27.5 months

72% of these tumors are high grade mammary intraepithelial neoplasias

the remaining 28% are invasive adenocarcinomas or adenosquamous carcinomas

Mouse Models of Human Disease	Note	Ref(s)
Models involving transgenes or other mutation types.¹
Breast Cancer		J:85799

¹Models involving transgenes or other mutation types may also appear in other sections of the table.

tg2

Tg(MMTV-TGFBR2)7Hlm/0

involves: C57BL/6 * DBA

tg3

Tg(MMTV-TGFBR2)7Hlm/?
Tg(MMTVTGFA)29Rjc/?

involves: C57BL/6 * DBA

Disease
models

Mouse Models of Human Disease	Note	Genotype			Ref(s)
			Allelic Composition	Genetic Background
Models involving transgenes or other mutation types.¹
Breast Cancer OMIM ID: 114480		tg1	Tg(MMTV-TGFBR2)7Hlm/Tg(MMTV-TGFBR2)7Hlm	involves: C57BL/6 * DBA	J:85799

¹Models involving transgenes or other mutation types may also appear in other sections of the table.

References

Original:	J:46432 Gorska AE et al., "Dominant-negative interference of the transforming growth factor beta type II receptor in mammary gland epithelium results in alveolar hyperplasia and differentiation in virgin mice." Cell Growth Differ 1998 Mar;9(3):229-38
All:	2 reference(s)

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