Mgl1<tm1Hed> Targeted Allele Detail MGI Mouse (MGI:2183546)

Mgl1^tm1Hed
Targeted Allele Detail

Nomenclature

Symbol:	Mgl1^tm1Hed
Name:	macrophage galactose N-acetyl-galactosamine specific lectin 1; targeted mutation 1, Stephen M Hedrick
MGI ID:	MGI:2183546
Synonyms:	mMGL^-/-
Gene:	Mgl1 Location: Chr11:69980125-69984336 bp, + strand Genetic Position: Chr11, 37.0 cM

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:78035
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	Exons 2 and 3 were replaced with a neomycin selection cassette. No transcript was detected in homozygous mutant mice via Northern blot and RT-PCR analyses, however, immunohistochemical staining showed low levels of reactivity in peritoneal exudate cells. (J:78035)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 3 strains available Cell Lines: 0 lines available
Carrying any Mgl1 Mutation:	4 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	cx2	cx3
hematopoietic system		√	√	√
increased erythrocyte cell number		√
increased mean corpuscular volume		√
increased hematocrit		√
increased hemoglobin content		√
decreased mean corpuscular hemoglobin		√
increased platelet cell number		√
decreased CD8-positive T cell number			√	√
		hm1	cx2	cx3
immune system		N	√	√
immune system phenotype		N
decreased CD8-positive T cell number			√	√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Mgl1^tm1Hed/Mgl1^tm1Hed	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
hematopoietic system increased erythrocyte cell number (J:78035) homozygotes display a slight but significant (3.5%) increase in red blood cell count relative to wild-type mice however, no changes are detected in red cell distribution width, and mutant erythrocyte life spans remain relatively unaffected as determined after biotinylation in vivo increased mean corpuscular volume (J:78035) homozygotes show only a slight (2.4%) increase in mean corpuscular volume relative to wild-type mice increased hematocrit (J:78035) homozygotes show a slight but significant (6.0 %) increase in hematocrit levels relative to wild-type mice increased hemoglobin content (J:78035) homozygotes show a slight but significant (3.8%) increase in hemoglobin content relative to wild-type mice decreased mean corpuscular hemoglobin (J:78035) homozygotes show a slight but significant (1.8%) decrease in mean corpuscular hemoglobin concentration relative to wild-type mice increased platelet cell number (J:78035) homozygotes show a 13.2% increase in platelet cell number relative to wild-type mice immune system immune system phenotype (J:78035) homozygotes exhibit a normal thymus as well as normal macrophage function and T- and B-cell development relative to control mice in addition, homozygotes show a normal cytotoxic T-cell response to major histocompatibility-disparate P815 tumor cells, with no differences in cytolytic activity, CD8 T cell expansion, and proportion of apoptotic (annexin V⁺) CD8 T cells relative to wild-type control mice
Genotype	Allelic Composition	Genetic Background
cx2	Mgl1^tm1Hed/Mgl1^tm1Hed St3gal1^tm1Jxm/St3gal1^tm1Jxm	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
hematopoietic system decreased CD8-positive T cell number (J:78035) although at 3 and 5 weeks of age, female (but not male) double homozygotes show a significant increase of CD8-positive T-cell numbers in the periphery relative to mice that are heterozygous for Mgl1^tm1Hed and homozygous for St3gal1^tm1Jxm, double homozygotes still exhibit a profound CD8 T-cell deficiency relative to wild-type mice immune system decreased CD8-positive T cell number (J:78035) although at 3 and 5 weeks of age, female (but not male) double homozygotes show a significant increase of CD8-positive T-cell numbers in the periphery relative to mice that are heterozygous for Mgl1^tm1Hed and homozygous for St3gal1^tm1Jxm, double homozygotes still exhibit a profound CD8 T-cell deficiency relative to wild-type mice
Genotype	Allelic Composition	Genetic Background
cx3	Mgl1^tm1Hed/Mgl1⁺ St3gal1^tm1Jxm/St3gal1^tm1Jxm	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
hematopoietic system decreased CD8-positive T cell number (J:78035) at 3 to 6 weeks of age, mutant mice exhibit a variable reduction in the percentage of CD8 T cells in peripheral blood relative to heterozygous St3gal1^tm1Jxm control mice (1-5% vs 11%, respectively) immune system decreased CD8-positive T cell number (J:78035) at 3 to 6 weeks of age, mutant mice exhibit a variable reduction in the percentage of CD8 T cells in peripheral blood relative to heterozygous St3gal1^tm1Jxm control mice (1-5% vs 11%, respectively)

References

Original:	J:78035 Onami TM et al., "Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins." Mol Cell Biol 2002 Jul;22(14):5173-81
All:	5 reference(s)

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