Lgals3<tm1Poi> Targeted Allele Detail MGI Mouse (MGI:2183057)

Lgals3^tm1Poi
Targeted Allele Detail

Nomenclature

Symbol:	Lgals3^tm1Poi
Name:	lectin, galactose binding, soluble 3; targeted mutation 1, Francoise Poirier
MGI ID:	MGI:2183057
Synonyms:	GaL3^-, galectin 3^-
Gene:	Lgals3 Location: Chr14:47987426-48005835 bp, + strand Genetic Position: Chr14, cytoband C1

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:47227
Parent Cell Line:	WW6 (ES Cell)
Strain of Origin:	STOCK 129/Sv and C57BL/6J and SJL

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	3.7kb of sequence, encompassing exons 2 through 4, was replaced via the insertion of a neomycin selection cassette. Immunostaining of fetal skin showed an absence of protein in homozygous mutant embryos. (J:47227)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 0 lines available
Carrying any Lgals3 Mutation:	4 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	hm2	hm3	cx4	cx5
cardiovascular system						√
decreased susceptibility to atherosclerosis						√
		hm1	hm2	hm3	cx4	cx5
hematopoietic system			√
decreased granulocyte number			√
		hm1	hm2	hm3	cx4	cx5
homeostasis/metabolism				√		√
decreased susceptibility to injury				√
increased circulating cholesterol level						√
		hm1	hm2	hm3	cx4	cx5
immune system			√	√
decreased granulocyte number			√
abnormal macrophage physiology				√
impaired macrophage recruitment				√
impaired macrophage phagocytosis				√
impaired neutrophil recruitment				√
increased interleukin-6 secretion				√
increased tumor necrosis factor secretion				√
lung inflammation				√
increased susceptibility to bacterial infection				√
sepsis				√
		hm1	hm2	hm3	cx4	cx5
life span/aging		N		√	N
life span/aging phenotype		N			N
increased sensitivity to induced morbidity/mortality				√
		hm1	hm2	hm3	cx4	cx5
limbs/digits/tail				√
abnormal long bone epiphyseal plate morphology				√
abnormal long bone hypertrophic chondrocyte zone				√
decreased width of hypertrophic chondrocyte zone				√
		hm1	hm2	hm3	cx4	cx5
renal/urinary system				√
abnormal kidney physiology				√
		hm1	hm2	hm3	cx4	cx5
reproductive system		N			N
reproductive system phenotype		N			N
		hm1	hm2	hm3	cx4	cx5
respiratory system				√
lung inflammation				√
		hm1	hm2	hm3	cx4	cx5
skeleton				√
abnormal long bone epiphyseal plate morphology				√
abnormal long bone hypertrophic chondrocyte zone				√
decreased width of hypertrophic chondrocyte zone				√
		hm1	hm2	hm3	cx4	cx5
skin/coat/nails		N			N
skin/coat/nails phenotype		N			N

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Lgals3^tm1Poi/Lgals3^tm1Poi	involves: 129 * 129/Sv * C57BL/6J * SJL
life span/aging life span-post-weaning/aging (J:47227) mice exhibit a normal lifespan reproductive system reproductive system phenotype (J:47227) mice exhibit normal reproduction skin/coat/nails skin/coat/nails phenotype (J:47227) mice exhibit normal skin structure
Genotype	Allelic Composition	Genetic Background
hm2	Lgals3^tm1Poi/Lgals3^tm1Poi	involves: 129/Sv * C57BL/6 * MF1 * SJL
hematopoietic system decreased granulocyte number (J:110422) while recruitment of granulocytes following treatment with thioglycolate is normal, granulocyte numbers 3 to 4 days after treatment are reduced 4-fold compared to in wild-type mice however, granulocyte apoptosis rates and phagocytosis of apoptotic granulocytes are normal immune system decreased granulocyte number (J:110422) while recruitment of granulocytes following treatment with thioglycolate is normal, granulocyte numbers 3 to 4 days after treatment are reduced 4-fold compared to in wild-type mice however, granulocyte apoptosis rates and phagocytosis of apoptotic granulocytes are normal
Genotype	Allelic Composition	Genetic Background
hm3	Lgals3^tm1Poi/Lgals3^tm1Poi	involves: 129/Sv * C57BL/6 * SJL
life span/aging increased sensitivity to induced morbidity/mortality (J:131407) mice infected with S. pneumoniae exhibit high mortality after 24 hours immune system abnormal macrophage physiology (J:131971) macrophage activation by IL-4 and IL-13 stimulation Is impaired however, macrophage activation by IFN-gamma and LPS is normal impaired macrophage recruitment (J:131971) when stimulated by IL-4 and IL-13 impaired macrophage phagocytosis (J:131407) phagocytosis of apoptotic neutrophils by bone marrow-derived macrophages is impaired however, phagocytosis of S. pneumonia is normal impaired neutrophil recruitment (J:131407) 15 hours after infection with S. pneumoniae however, myeloperoxidase activity is similar to in wild-type mice 15 hours after infection with S. pneumoniae increased interleukin-6 secretion (J:131407) 15 hours after infection with S. pneumoniae, IL-6 concentration in bronchoalveolar lavage is increased compared to in wild-type mice increased tumor necrosis factor secretion (J:131407) 15 hours after infection with S. pneumoniae, TNF-alpha concentration in bronchoalveolar lavage is increased compared to in wild-type mice lung inflammation (J:131407) after 15 hours, mice infected with S. pneumoniae exhibit increased cell infiltrate in the lungs and develop severe pneumonia with increased lung injury and septicemia compared to wild-type mice increased susceptibility to bacterial infection (J:131407) mice infected with S. pneumoniae exhibit high mortality after 24 hours after 15 hours, mice infected with S. pneumoniae exhibit increased cell infiltrate but decreased total cell counts in the lungs and develop severe pneumonia with increased lung injury and septicemia compared to wild-type mice after 15 hours, mice infected with S. pneumoniae exhibit a 450-fold increase in bacterial load compared to wild-type mice and blood cultures indicate 100 % bacteremia compared to 30% in wild-type mice sepsis (J:131407) after 15 hours, mice infected with S. pneumoniae exhibit septicemia unlike wild-type mice skeleton abnormal long bone epiphyseal plate morphology (J:66902) unlike in wild-type mice, a large zone of empty lacunae is observed between the last row of hypertrophic cells and the vascular invasion front abnormal long bone hypertrophic chondrocyte zone (J:66902) chondrocytes within the hypertrophic zone are larger and more vacuolated than in wild-type mice mice exhibit an increased number of nonhypertrophic cells in the late hyprtrophic zone compared to in wild-type mice chondrocytes in the zone contain unusually large intracellular glycogen aggregates decreased width of hypertrophic chondrocyte zone (J:66902) the hypertrophic zone is reduced in size and contains 20% fewer hypertrophic cells than in wild type mice renal/urinary system abnormal kidney physiology (J:131371) mice exhibit less renal fibrosis following unilateral ureter obstruction (UUO) compared to wild-type mice however, macrophage recruitment and cytokine production induced by UUO is normal respiratory system lung inflammation (J:131407) after 15 hours, mice infected with S. pneumoniae exhibit increased cell infiltrate in the lungs and develop severe pneumonia with increased lung injury and septicemia compared to wild-type mice homeostasis/metabolism decreased susceptibility to injury (J:131371) mice exhibit less renal fibrosis following unilateral ureter obstruction (UUO) compared to wild-type mice however, macrophage recruitment and cytokine production induced by UUO is normal limbs/digits/tail abnormal long bone epiphyseal plate morphology (J:66902) unlike in wild-type mice, a large zone of empty lacunae is observed between the last row of hypertrophic cells and the vascular invasion front abnormal long bone hypertrophic chondrocyte zone (J:66902) chondrocytes within the hypertrophic zone are larger and more vacuolated than in wild-type mice mice exhibit an increased number of nonhypertrophic cells in the late hyprtrophic zone compared to in wild-type mice chondrocytes in the zone contain unusually large intracellular glycogen aggregates decreased width of hypertrophic chondrocyte zone (J:66902) the hypertrophic zone is reduced in size and contains 20% fewer hypertrophic cells than in wild type mice
Genotype	Allelic Composition	Genetic Background
cx4	Lgals1^tm1Rob/Lgals1^tm1Rob Lgals3^tm1Poi/Lgals3^tm1Poi	involves: 129 * 129/Sv * 129S/SvEv * C57BL/6J * SJL
life span/aging life span-post-weaning/aging (J:47227) mice exhibit a normal lifespan reproductive system reproductive system phenotype (J:47227) mice exhibit normal reproduction skin/coat/nails skin/coat/nails phenotype (J:47227) mice exhibit normal skin structure
Genotype	Allelic Composition	Genetic Background
cx5	Apoe^tm1Unc/Apoe^tm1Unc Lgals3^tm1Poi/Lgals3^tm1Poi	involves: 129/Sv * C57BL/6 * SJL
cardiovascular system decreased susceptibility to atherosclerosis (J:130923) at 25 to 31 weeks of age, mice exhibit a lower number inflammatory infiltrate and mast cells in adventitia than Apoe^tm1Unc homozygotes at 36 to 44 months of age, mice exhibit fewer atherosclerotic lesions and atheromatous plaques compared to in Apoe^tm1Unc homozygotes and do not exhibit an age-related increase in lesion number, athlerosclerotic microaneurysms or periaortic vascular channels that are evident in Apoe^tm1Unc homozygotes homeostasis/metabolism increased circulating cholesterol level (J:130923) compared to in Lgals3^tm1Poi homozygotes

References

Original:	J:47227 Colnot C et al., "Embryonic implantation in galectin 1/galectin 3 double mutant mice." Dev Dyn 1998 Apr;211(4):306-13
All:	13 reference(s)

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