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| Nomenclature |
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Symbol:
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Dldtm1Ptl
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Name:
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dihydrolipoamide dehydrogenase;
targeted mutation 1, Mulchand S Patel
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MGI ID: |
MGI:2182403 |
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Synonyms: |
Dld-, Dldtm1mjp |
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Gene:
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Dld
Location:
Chr12:32016429-32036263 bp, - strand
Genetic Position: Chr12,
15.1 cM
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Mutation
origin |
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Germline Transmission:
| Earliest citation of germline transmission:
J:45074
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Parent Cell Line:
| E14.1 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: |
Targeted (knock-out) |
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Mutation: |
Disruption caused by insertion of vector |
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Exon 10 was disrupted by the insertion of a neomycin selection cassette. Northern blot analysis of total hepatic and renal RNA showed that transcript levels are reduced by approximately 50% in heterozygous mice. RT-PCR analysis confirmed that the neo transgene was not excised via an altered splicing pattern.
(J:45074) |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
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Key:
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| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
 |
expected model not found |
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Phenotypic
data by
genotype
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Phenotypic Data by Genotype
(show or
hide all phenotypic details)
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| Genotype | Allelic Composition | Genetic Background |
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  hm1
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Dldtm1Ptl/Dldtm1Ptl |
involves: 129P2/OlaHsd * Black Swiss |
lethality/prenatal-perinatal embryogenesis growth/size |
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| References |
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Original: |
J:45074
Johnson MT et al.,
"Targeted disruption of the murine dihydrolipoamide dehydrogenase gene (Dld) results in perigastrulation lethality."
Proc Natl Acad Sci U S A 1997 Dec 23;94(26):14512-7
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All: |
4 reference(s)
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