Lgals1<tm1Rob> Targeted Allele Detail MGI Mouse (MGI:2181809)

Lgals1^tm1Rob
Targeted Allele Detail

Nomenclature

Symbol:	Lgals1^tm1Rob
Name:	lectin, galactose binding, soluble 1; targeted mutation 1, Elizabeth J Robertson
MGI ID:	MGI:2181809
Synonyms:	gal-1^-, gal1^-
Gene:	Lgals1 Location: Chr15:78757172-78760622 bp, + strand Genetic Position: Chr15, 44.9 cM, cytoband E

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:16245
Parent Cell Line:	CCE/EK.CCE (ES Cell)
Strain of Origin:	129S/SvEv-Gpi1^c

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	Exon 2, encoding the carbohydrate-binding domain, was replaced with a neomycin selection cassette. Western blot analysis and immunostaining of sections showed an absence of encoded protein in muscle tissue obtained from adult homozygous mutant mice. (J:16245)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 0 lines available
Carrying any Lgals1 Mutation:	9 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	hm2	hm3	cx4	cx5
behavior/neurological		√
increased thermal nociceptive threshold		√
		hm1	hm2	hm3	cx4	cx5
immune system		√	√
increased susceptibility to experimental autoimmune encephalomyelitis		√
abnormal cytokine secretion			√
increased interferon-gamma secretion		√
increased interleukin-2 secretion		√
		hm1	hm2	hm3	cx4	cx5
life span/aging					N
life span/aging phenotype					N
		hm1	hm2	hm3	cx4	cx5
muscle		√				√
delayed muscle development		√				√
abnormal skeletal muscle fiber morphology						√
decreased skeletal muscle fiber diameter						√
abnormal muscle regeneration						√
		hm1	hm2	hm3	cx4	cx5
nervous system		√		√
abnormal postnatal subventricular zone morphology		√
abnormal neuromuscular synapse morphology		√
abnormal sensory neuron morphology		√
abnormal olfactory neuron morphology		√
abnormal mechanoreceptor morphology		√
abnormal nociceptor morphology		√
abnormal dorsal root ganglion morphology		√
abnormal olfactory nerve morphology				√
		hm1	hm2	hm3	cx4	cx5
reproductive system			N		N
reproductive system phenotype			N		N
		hm1	hm2	hm3	cx4	cx5
skin/coat/nails					N
skin/coat/nails phenotype					N
		hm1	hm2	hm3	cx4	cx5
touch/vibrissae		√
increased thermal nociceptive threshold		√
		hm1	hm2	hm3	cx4	cx5
normal phenotype				N
no abnormal phenotype detected				N

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Lgals1^tm1Rob/Lgals1^tm1Rob	involves: 129S/SvEv
nervous system abnormal postnatal subventricular zone morphology (J:109590) mice exhibit fewer proliferating cells and slowly dividing cells in the adult subventricular zone (SVZ) compared to in wild-type mice fewer progenitor cells are found in the adult SVZ compared to in wild-type mice however, the number of apoptotic cells in the SVZ is normal abnormal neuromuscular synapse morphology (J:122104) mice exhibit a delay in eliminating nerve endings after axotomy compared to in wild-type mice abnormal sensory neuron morphology (J:101850) mice exhibit more myelinated sensory axons in the dorsal root ganglia than in wild-type mice abnormal olfactory neuron morphology (J:36694) olfactory axons exhibit abnormal projections in the dorsocaudal olfactory bulb and, unlike in wild-type mice most axons do not terminate in discrete glomeruli abnormal mechanoreceptor morphology (J:101850) mice exhibit fewer IB4-binding neurons (non-peptidergic nociceptor neurons) in the dorsal root ganglia than in wild-type mice IB4-binding axons extend deeper into the dorsal horn of the spinal cord than in wild-type mice abnormal nociceptor morphology (J:101850) mice exhibit more myelinated sensory axons in the dorsal root ganglia than in wild-type mice abnormal dorsal root ganglion morphology (J:101850) mice exhibit fewer IB4-binding neurons (non-peptidergic nociceptor neurons), more NF200-exressing neurons (large-diameter mechanoreceptor neurons) and more myelinated sensory axons in the dorsal root ganglia than in wild-type mice the mean diameter of neurons in the dorsal root ganglia is reduced compared to in wild-type mice however, the total number of neurons in the dorsal root ganglia is normal immune system increased interferon-gamma secretion (J:123416) stimulated CD4+ T cells secrete more IFN-gamma than wild-type mice increased interleukin-2 secretion (J:123416) stimulated CD4+ T cells secrete more IL-2 than wild-type mice increased susceptibility to experimental autoimmune encephalomyelitis (J:123416) when treated to induce experimental autoimmune encephalomyelitis, mice exhibit a more severe disease, vigorous splenocyte proliferation, IL-17 and IFN-gamma production and a greater accumulation of MOG35-55-specific TH1 and TH-17 cells compared to in similarly treated wild-type mice muscle delayed muscle development (J:119480) mice exhibit a delay in muscle development beginning at day 1 through day 7 touch/vibrissae increased thermal nociceptive threshold (J:101850) behavior/neurological increased thermal nociceptive threshold (J:101850)
Genotype	Allelic Composition	Genetic Background
hm2	Lgals1^tm1Rob/Lgals1^tm1Rob	involves: 129S/SvEv * 129S1/SvImJ * DBA/2J
immune system abnormal cytokine secretion (J:130208) following treatment with dydrogesterone, uterine support cells exhibit an increase in TH1 to TH2 cytokine ratio compared to wild-type mice reproductive system reproductive system phenotype (J:130208) mice exhibit more fetal loss than in wild-type mice without altered number of implantation sites or litter size
Genotype	Allelic Composition	Genetic Background
hm3	Lgals1^tm1Rob/Lgals1^tm1Rob	involves: 129S/SvEv * MF1
nervous system abnormal olfactory nerve morphology (J:36694) although the olfactory nerve itself was normal, projections from it to the dorsocaudal surface of the olfactory bulb were severly reduced and axons never terminated in glomeruli mice were otherwise normal normal phenotype no abnormal phenotype detected (J:16245) mice are normal
Genotype	Allelic Composition	Genetic Background
cx4	Lgals1^tm1Rob/Lgals1^tm1Rob Lgals3^tm1Poi/Lgals3^tm1Poi	involves: 129 * 129/Sv * 129S/SvEv * C57BL/6J * SJL
life span/aging life span-post-weaning/aging (J:47227) mice exhibit a normal lifespan reproductive system reproductive system phenotype (J:47227) mice exhibit normal reproduction skin/coat/nails skin/coat/nails phenotype (J:47227) mice exhibit normal skin structure
Genotype	Allelic Composition	Genetic Background
cx5	Lgals1^tm1Rob/Lgals1^tm1Rob Myf5^tm1Pas/Myf5⁺	involves: 129S/SvEv * 129S2/SvPas
muscle delayed muscle development (J:119480) mice exhibit a delay in muscle development cultured myoblasts exhibit impaired fusion compared to in wild-type myoblasts abnormal skeletal muscle fiber morphology (J:119480) myonuclear numbers are decreased decreased skeletal muscle fiber diameter (J:119480) adult mice exhibit a 22% reduction in muscle fiber diameter abnormal muscle regeneration (J:119480) muscle regeneration is impaired and mice produce more smaller diameter fibers than in wild-type mice

References

Original:	J:16245 Poirier F et al., "Normal development of mice carrying a null mutation in the gene encoding the L14 S-type lectin." Development 1993 Dec;119(4):1229-36
All:	25 reference(s)

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