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| Nomenclature |
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Symbol:
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Akt2tm1.1Mbb
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Name:
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thymoma viral proto-oncogene 2;
targeted mutation 1.1, Morris J Birnbaum
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MGI ID: |
MGI:2158456 |
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Synonyms: |
Akt2 KO, Akt2- |
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Gene:
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Akt2
Location:
Chr7:28376571-28425845 bp, + strand
Genetic Position: Chr7,
6.5 cM, cytoband B1
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Mutation
origin |
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Germline Transmission:
| Earliest citation of germline transmission:
J:71491
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Parent Cell Line:
| E14 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: |
Targeted (knock-out) |
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Mutations: |
Insertion, Intragenic deletion |
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This allele is a derivative of Akt2tm1Mbb. Cre-mediated recombination in vivo under the control of a 6 kb 5'-flanking sequence from the Pou3f4 gene excised the floxed exons 4 and 5 in the germline. The excision of exons 4 and 5 results in a frameshift mutation that would lead to a premature termination even if the remaining exon 3 were to splice to exon 6. Exon 5 encodes the lysine residue necessary for catalytic activity. Western blot analyses using a polyclonal antibody did not detect protein in liver, muscle, and isolated adipocytes from homozygous mice. (J:71491) |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
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Key:
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| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
 |
expected model not found |
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Phenotypic
data by
genotype
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Phenotypic Data by Genotype
(show or
hide all phenotypic details)
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Disease
models
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| References |
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Original: |
J:71491
Cho H et al.,
"Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta)."
Science 2001 Jun 1;292(5522):1728-31
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All: |
20 reference(s)
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