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| Nomenclature |
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Symbol:
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Trp53tm1Holl
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Name:
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transformation related protein 53;
targeted mutation 1, Monica Hollstein
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MGI ID: |
MGI:2157167 |
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Synonyms: |
HUPKI, p5372arg, p53hki, p53KI |
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Gene:
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Trp53
Location:
Chr11:69393861-69405375 bp, + strand
Genetic Position: Chr11,
39.0 cM, cytoband B2-C
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Mutation
origin |
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Germline Transmission:
| Earliest citation of germline transmission:
J:68918
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Parent Cell Line:
| E14.1 (ES Cell) |
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Strain of Origin:
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129P2/OlaHsd
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Mutation
description |
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Allele
Type: |
Targeted (knock-in) |
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Mutations: |
Insertion, Intragenic deletion |
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Insertion of a loxP-flanked neomycin-thymidine kinase expression cassette replaced the mouse DNA-binding domain (exons 4-9) with the corresponding homologous segment of the normal human gene, leaving transcription under control of the endogenous promoter. Cre-mediated recombination in ES cells excised the neo-tk sequences. cDNA sequencing studies determined that transcripts from spleen of homozygous mutant mice carried the human sequence for exons 4-9, and that the gene is correctly spliced and transcribed. (J:68918) |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
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Key:
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| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
 |
expected model not found |
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Phenotypic
data by
genotype
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Phenotypic Data by Genotype
(show or
hide all phenotypic details)
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| References |
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Original: |
J:68918
Luo JL et al.,
"Knock-in mice with a chimeric human/murine p53 gene develop normally and show wild-type p53 responses to DNA damaging agents: a new biomedical research tool."
Oncogene 2001 Jan 18;20(3):320-8
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All: |
7 reference(s)
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