Timp2<tm1Pds> Targeted Allele Detail MGI Mouse (MGI:1888708)

Timp2^tm1Pds
Targeted Allele Detail

Nomenclature

Symbol:	Timp2^tm1Pds
Name:	tissue inhibitor of metalloproteinase 2; targeted mutation 1, Paul D Soloway
MGI ID:	MGI:1888708
Synonyms:	TIMP-2KO, Timp2-, TIMP2KO
Gene:	Timp2 Location: Chr11:118162383-118217054 bp, - strand Genetic Position: Chr11, 72.0 cM

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:64001
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	Replacement of the first coding exon with a neomycin cassette. (J:64001)
Inheritance:	Recessive

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Timp2 Mutation:	1 strain or line available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	hm2
behavior/neurological		√
abnormal cued conditioning behavior		√
		hm1	hm2
hematopoietic system			√
increased neutrophil cell number			√
		hm1	hm2
homeostasis/metabolism			√
abnormal enzyme/coenzyme activity			√
		hm1	hm2
immune system			√
increased neutrophil cell number			√
		hm1	hm2
nervous system		√
decreased prepulse inhibition		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Timp2^tm1Pds/Timp2^tm1Pds	B6.129S4-Timp2^tm1Pds
behavior/neurological abnormal cued conditioning behavior (J:99752) null mice have a deficit in fear-potentiated startle (FPS) vs wild-type; mice generate greater startle responses compared to wild-type mice in presence of tone before fear conditioning (ie. P0 - first testing session) all males show an increase in FPS initially (from P0 to P1) but FPS decreases from that point in knockout and knockdown mice; in wild-type and heterozygotes, amplitudes of startle responses continue to increase wild-type and heterozygous males acquire conditioned fear over the course of the test, but null and knockdown mice do not females do not show any proportional increases in FPS nervous system decreased prepulse inhibition (J:99752) Timp2-null mice have significantly reduced prepulse inhibition (PPI)
Genotype	Allelic Composition	Genetic Background
hm2	Timp2^tm1Pds/Timp2^tm1Pds	involves: 129S4/SvJae * C57BL/6
hematopoietic system increased neutrophil cell number (J:112941) at day 7 after treatment, BAL neutrophil percentage and concentration are 3- and 10-fold higher in Timp1-null mice compared to Timp2-null animals; values for Timp2-null mice are similar to wild-type immune system increased neutrophil cell number (J:112941) at day 7 after treatment, BAL neutrophil percentage and concentration are 3- and 10-fold higher in Timp1-null mice compared to Timp2-null animals; values for Timp2-null mice are similar to wild-type homeostasis/metabolism abnormal enzyme/coenzyme activity (J:64001) activation of proMMP-2 in vivo is dramatically altered with Timp2 deficiency

References

Original:	J:64001 Wang Z et al., "TIMP-2 is required for efficient activation of proMMP-2 in vivo" J Biol Chem 2000 Aug 25;275(34):26411-5
All:	10 reference(s)

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