Csk<tm1Sor> Targeted Allele Detail MGI Mouse (MGI:1861914)

Csk^tm1Sor
Targeted Allele Detail

Nomenclature

Symbol:	Csk^tm1Sor
Name:	c-src tyrosine kinase; targeted mutation 1, Philippe Soriano
MGI ID:	MGI:1861914
Synonyms:	Csk-
Gene:	Csk Location: Chr9:57474453-57492987 bp, - strand Genetic Position: Chr9, 32.0 cM

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:12622
Parent Cell Line:	AB1 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1⁺

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	Deletion of all coding sequences and replacement with a neomycin cassette. (J:12622)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Csk Mutation:	6 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

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Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1
cardiovascular system		√
poor circulation		√
		hm1
craniofacial		√
megacephaly		√
		hm1
embryogenesis		√
incomplete embryo turning		√
caudal body truncation		√
decreased embryo size		√
incomplete cephalic closure		√
open neural tube		√
wavy neural tube		√
absent notochord		√
abnormal allantois morphology		√
enlarged allantois		√
		hm1
growth/size		√
decreased embryo size		√
		hm1
lethality/prenatal-perinatal		√
embryonic lethality during organogenesis		√
		hm1
nervous system		√
incomplete cephalic closure		√
open neural tube		√
wavy neural tube		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype

Allelic Composition

Genetic Background

hm1

Csk^tm1Sor/Csk^tm1Sor

either: (involves: 129S7/SvEvBrd) or (involves: 129S7/SvEvBrd * C57BL/6J)

lethality/prenatal-perinatalembryonic lethality during organogenesis (J:12622)

most homozygotes die between E9.5 and E10.5

no homozygotes are recovered at or after E11.5

embryogenesisincomplete embryo turning (J:12622)

at E9.5, most homozygous mutant embryos remain unturned

caudal body truncation (J:12622)

at E9.5, homozygotes exhibit a small and underdeveloped caudal portion

decreased embryo size (J:12622)

at E9.5, most homozygous mutant embryos are ~25% to ~50% the size of wild-type embryos

incomplete cephalic closure (J:12622)

at E9.5, homozygotes fail to close their cephalic neural folds

open neural tube (J:12622)

at E9.5, the mutant neural tube is closed at the level of the otic vesicle but remains open beyond the midbrain-forebrain boundary

abnormal folding of the neuroectoderm is noted at the level of the cephalic folds and of the neural tube

wavy neural tube (J:12622)

at E9.5, homozygotes exhibit a wavy and disorganized neural tube

in addition, the neural tube is often curved and infiltrated with pyknotic cells

absent notochord (J:12622)

at E8.5, the mutant notochord is consistently smaller or even absent

abnormal allantois morphology (J:12622)

at E9.5, the allantois is often abnormal and either resorbing or tuberous

enlarged allantois (J:12622)

allantois is often overgrown with a "bleb" at its extremity

nervous systemincomplete cephalic closure (J:12622)

at E9.5, homozygotes fail to close their cephalic neural folds

open neural tube (J:12622)

at E9.5, the mutant neural tube is closed at the level of the otic vesicle but remains open beyond the midbrain-forebrain boundary

abnormal folding of the neuroectoderm is noted at the level of the cephalic folds and of the neural tube

wavy neural tube (J:12622)

at E9.5, homozygotes exhibit a wavy and disorganized neural tube

in addition, the neural tube is often curved and infiltrated with pyknotic cells

growth/sizedecreased embryo size (J:12622)

at E9.5, most homozygous mutant embryos are ~25% to ~50% the size of wild-type embryos

cardiovascular systempoor circulation (J:12622)

at E9.5, blood circulation is essentially absent in the visceral yolk sac and in mutant embryos

however, all homozygotes at this stage show normal decidual sizes and normal beating of the heart

craniofacialmegacephaly (J:12622)

at E9.5, homozygotes display a disproportionately bulky and dilated head

References

Original:	J:12622 Imamoto A et al., "Disruption of the csk gene, encoding a negative regulator of Src family tyrosine kinases, leads to neural tube defects and embryonic lethality in mice." Cell 1993 Jun 18;73(6):1117-24
All:	3 reference(s)

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