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| Nomenclature |
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Symbol:
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Rac2tm1Mddw
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Name:
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RAS-related C3 botulinum substrate 2;
targeted mutation 1, Mary C Dinauer and David A Williams
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MGI ID: |
MGI:1857701 |
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Synonyms: |
rac2-, Rac2tm1Daw |
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Gene:
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Rac2
Location:
Chr15:78391308-78403150 bp, - strand
Genetic Position: Chr15,
Syntenic
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Mutation
origin |
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Germline Transmission:
| Earliest citation of germline transmission:
J:53362
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Parent Cell Line:
| CCE/EK.CCE (ES Cell) |
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Strain of Origin:
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129S/SvEv-Gpi1c
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Mutation
description |
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Allele
Type: |
Targeted (knock-out) |
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Mutations: |
Insertion, Intragenic deletion |
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A neomycin resistance cassette replaced a genomic fragment containing exon 1, which encodes the translational initiation site and part of the GTP-binding domain. Northern blot and RT-PCR analysis demonstrated that no transcripts were detectable in total RNA derived from neutrophils or lymphocytes in homozygous mice. Western blot analysis confirmed an absence of protein produced from this allele in peritoneal exudate cells from homozygous mice. (J:53362) |
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| Find Mice (IMSR) |
Mouse strains and cell lines available from the
International Mouse Strain Resource
(IMSR)
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Phenotype
summary
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Phenotype Summary by Mammalian Phenotype terms
(show or
hide all annotated terms)
Genotypes are listed in the next section.
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Key:
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| hm |
homozygous |
ht |
heterozygous |
| cn |
conditional genotype |
cx |
complex: > 1 genome feature |
| tg |
involves transgenes |
ot |
other: hemizygous, indeterminate,... |
| N |
normal phenotype |
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expected model not found |
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Phenotypic
data by
genotype
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Phenotypic Data by Genotype
(show or
hide all phenotypic details)
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| References |
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Original: |
J:53362
Roberts AW et al.,
"Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is characterized by abnormalities in neutrophil function and host defense."
Immunity 1999 Feb;10(2):183-96
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All: |
38 reference(s)
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