Plaur<tm1Jld> Targeted Allele Detail MGI Mouse (MGI:1857290)

Plaur^tm1Jld
Targeted Allele Detail

Nomenclature

Symbol:	Plaur^tm1Jld
Name:	plasminogen activator, urokinase receptor; targeted mutation 1, Jay L Degen
MGI ID:	MGI:1857290
Synonyms:	Plaur^tm1, uPAR^-
Gene:	Plaur Location: Chr7:25247519-25260893 bp, + strand Genetic Position: Chr7, cytoband A3

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:26874
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	Exon 3, encoding the second half of the amino-terminal uPA binding domain, was replaced by an HPRT minigene. (J:26874)
Inheritance:	Recessive

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Plaur Mutation:	2 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	hm2	hm3	tg4
cardiovascular system				√	√
decreased angiogenesis				√
cardiac fibrosis					√
heart inflammation					√
		hm1	hm2	hm3	tg4
homeostasis/metabolism			N
homeostasis/metabolism phenotype			N
		hm1	hm2	hm3	tg4
immune system		√			√
abnormal macrophage physiology		√
heart inflammation					√
		hm1	hm2	hm3	tg4
renal/urinary system		N
renal/urinary system phenotype		N

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Plaur^tm1Jld/Plaur^tm1Jld	involves: 129P2/OlaHsd * Black Swiss
renal/urinary system renal/urinary system phenotype (J:26874) homozygotes exhibit a redistribution of urokinase plasminogen activator (uPA) in some tissues but show no changes in the accumulation or proteolytic activation of uPA in the urogenital tract as in wild-type mice, most of the uPA in the urine of mutant mice is in the two-chain form immune system abnormal macrophage physiology (J:26874) activated peritoneal macrophages from mutant mice fail to promote cell-surface plasminogen activation in vitro; however, homozygotes are viable, fertile and survive to adulthood with no apparent anatomical defects also, homozygotes that are unchallenged by disease, infection or injury show no pathological lesions or hepatic fibrin deposits up to 6-8 months of age
Genotype	Allelic Composition	Genetic Background
hm2	Plaur^tm1Jld/Plaur^tm1Jld	involves: 129P2/OlaHsd * C57BL/6
homeostasis/metabolism homeostasis/metabolism phenotype (J:63134) 14 days after bleomycin treatment, wild-type and mutant mice display a similar increase in lung collagen (hydroxyproline) content 14 days after bleomycin treatment, wild-type and mutant mice show similar focal areas of pulmonary fibrosis surrounded by normal alveoli; no intra-alveolar hemorrhage or early deaths are observed
Genotype	Allelic Composition	Genetic Background
hm3	Plaur^tm1Jld/Plaur^tm1Jld	involves: 129P2/OlaHsd * C57BL/6J
cardiovascular system decreased angiogenesis (J:108675) aortic vessel explants show a reduced rate of sprouting (2/3 of controls) in collagen lattices
Genotype	Allelic Composition	Genetic Background
tg4	Plaur^tm1Jld/Plaur^tm1Jld Tg(MSR1-Plau)1Ddi/0	B6.Cg-Plaur^tm1Jld Tg(MSR1-Plau)1Ddi
cardiovascular system cardiac fibrosis (J:113474) exhibit more fibrosis at 15 weeks of age than single Plaur homozygotes although show a similar degree of fibrosis as seen in single Tg(MSR1-Plau)1Ddi mutants heart inflammation (J:113474) exhibit significantly more cardiac macrophages at 15 weeks of age than single Plaur homozygotes, however show a similar accumulation as seen in single Tg(MSR1-Plau)1Ddi mutants immune system heart inflammation (J:113474) exhibit significantly more cardiac macrophages at 15 weeks of age than single Plaur homozygotes, however show a similar accumulation as seen in single Tg(MSR1-Plau)1Ddi mutants

References

Original:	J:26874 Bugge TH et al., "The receptor for urokinase-type plasminogen activator is not essential for mouse development or fertility." J Biol Chem 1995 Jul 14;270(28):16886-94
All:	15 reference(s)

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