Itga5<tm1Hyn> Targeted Allele Detail MGI Mouse (MGI:1857207)

Itga5^tm1Hyn
Targeted Allele Detail

Nomenclature

Symbol:	Itga5^tm1Hyn
Name:	integrin alpha 5 (fibronectin receptor alpha); targeted mutation 1, Richard Hynes
MGI ID:	MGI:1857207
Synonyms:	alpha5-, alpha5-null
Gene:	Itga5 Location: Chr15:103174720-103197194 bp, - strand Genetic Position: Chr15, 57.4 cM

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:16248
Parent Cell Line:	D3 (ES Cell)
Strain of Origin:	129S2/SvPas

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	A neomycin resistance cassette replaced 330 bp of the gene, deleting most of the first exon. (J:16248)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Itga5 Mutation:	11 strains or lines available

Expression

In Mice Carrying this Mutation:

44 assay results

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	cx2
behavior/neurological			√
abnormal spatial learning			√
		hm1	cx2
cardiovascular system		√
abnormal blood vessel morphology		√
abnormal dorsal aorta morphology		√
		hm1	cx2
cellular		N
cellular phenotype		N
		hm1	cx2
embryogenesis		√
incomplete embryo turning		√
abnormal rostral-caudal axis patterning		√
abnormal embryonic tissue morphology		√
abnormal mesoderm development		√
abnormal paraxial mesoderm		√
kinked neural tube		√
failure of somite differentiation		√
abnormal extraembryonic tissue morphology		√
abnormal vitelline vasculature		√
embryonic growth retardation		√
		hm1	cx2
growth/size		√
embryonic growth retardation		√
		hm1	cx2
lethality/prenatal-perinatal		√
embryonic lethality during organogenesis		√
		hm1	cx2
nervous system		√	√
kinked neural tube		√
reduced long term potentiation			√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Itga5^tm1Hyn/Itga5^tm1Hyn	involves: 129S2/SvPas * C57BL/6J
lethality/prenatal-perinatal embryonic lethality during organogenesis (J:16248) homozygotes die between E10.0 and E11.0 embryogenesis incomplete embryo turning (J:16248) at E9.5, mutant embryos are only partially turned into the fetal position abnormal rostral-caudal axis patterning (J:16248) at E8.5, homozygotes display an abnormal curvature and a slightly truncated anterior-posterior axis at E9.5, the posterior trunk of mutant embryos is deformed and only 1/3 of E9.0 wild-type length although delayed, the anterior region (including heads, branchial arches and heart) appears relatively unaffected abnormal embryonic tissue morphology (J:16248) abnormal mesoderm development (J:16248) at E9.5, the extraembryonic mesodermal layer is reduced and separated from the extraembryonic endoderm abnormal paraxial mesoderm (J:16248) at E9.5, homozygotes display a deficit in paraxial mesoderm in the region flanking the neural tube mesenchymeal cells, normally found on the dorsal side of somites are absent kinked neural tube (J:16248) at E9.5, homozygotes exhibit kinked neural tubes in the posterior trunk region failure of somite differentiation (J:16248) at E9.5, mutant embryos lack somites in the truncated posterior regions in contrast, normal somite numbers (7-10 pairs) are noted anteriorly at E8.0-E8.5, most mutant embryos exhibit normal numbers of somites relative to wild-type embryos (4-13 pairs) abnormal extraembryonic tissue morphology (J:16248) abnormal vitelline vasculature (J:16248) at E9.5, mutant yolk sacs display abnormal blood vessels, with large numbers of blood cells leaking into the exocoelomic space embryonic growth retardation (J:16248) at E9.5, mutant embryos are developmentally retarded and equivalent in size to E9.0 wild-type embryos by E10.5, mutant embryos are equivalent in size to E9.5 wild-type embryos growth/size embryonic growth retardation (J:16248) at E9.5, mutant embryos are developmentally retarded and equivalent in size to E9.0 wild-type embryos by E10.5, mutant embryos are equivalent in size to E9.5 wild-type embryos nervous system kinked neural tube (J:16248) at E9.5, homozygotes exhibit kinked neural tubes in the posterior trunk region cardiovascular system abnormal blood vessel morphology (J:16248) all homozygotes form hearts and a vascular system; however, primitive blood vessels are distended and leaky at E9.5, homozygotes show vascular defects both in the embryo and in extraembryonic vasculature, with fewer blood cells present in the heart and blood vessels abnormal dorsal aorta morphology (J:16248) at E9.5, the mutant dorsal aortae are not fully closed, with leakage of primitive blood cells into the mesoderm-deficient space cellular cellular phenotype (J:16248) mutant embryonic fibroblasts are able to assemble fibronectin matrix, form focal contacts, and migrate on fibronectin
Genotype	Allelic Composition	Genetic Background
cx2	Itga3^tm1Jak/Itga3⁺ Itga5^tm1Hyn/Itga5⁺ Itga8^tm1Lfr/Itga8⁺	involves: 129S2/SvPas * 129S4/SvJae * 129X1/SvJ * C57BL/6J
nervous system reduced long term potentiation (J:84858) nearly complete elimination of long term potentiation after 60 minutes behavior/neurological abnormal spatial learning (J:84858) deficient in spatial memory in a Morris water maze test normal fear conditioning however

References

Original:	J:16248 Yang JT et al., "Embryonic mesodermal defects in alpha 5 integrin-deficient mice." Development 1993 Dec;119(4):1093-105
All:	6 reference(s)

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