Itga4<tm1Hyn> Targeted Allele Detail MGI Mouse (MGI:1857206)

Itga4^tm1Hyn
Targeted Allele Detail

Nomenclature

Symbol:	Itga4^tm1Hyn
Name:	integrin alpha 4; targeted mutation 1, Richard Hynes
MGI ID:	MGI:1857206
Synonyms:	alpha4-null, alpha4KO
Gene:	Itga4 Location: Chr2:79095583-79173280 bp, + strand Genetic Position: Chr2, 46.0 cM

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:23140
Parent Cell Line:	D3 (ES Cell)
Strain of Origin:	129S2/SvPas

Mutation
description

Allele Type:	Targeted (knock-out)
Mutations:	Insertion, Intragenic deletion
	A neomycin resistance cassette replaced a 950 bp fragment which contained most of the first exon of the gene. (J:23140)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)

Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Itga4 Mutation:	3 strains or lines available

Phenotype
summary

Phenotype Summary by Mammalian Phenotype terms

(show or hide all annotated terms)

Genotypes are listed in the next section.

Key:

hm	homozygous	ht	heterozygous
cn	conditional genotype	cx	complex: > 1 genome feature
tg	involves transgenes	ot	other: hemizygous, indeterminate,...
N	normal phenotype		expected model not found

Affected Systems	Genotypes:	hm1	ht2
cardiovascular system		√	√
abnormal cardiovascular system morphology		√
abnormal coronary artery morphology		√
abnormal epicardium morphology		√	√
heart hemorrhage		√
		hm1	ht2
craniofacial		√
abnormal craniofacial morphology		√
		hm1	ht2
embryogenesis		√
abnormal extraembryonic tissue morphology		√
dilated allantois		√
abnormal placenta morphology		√
failure of chorioallantoic fusion		√
		hm1	ht2
lethality/prenatal-perinatal		√
embryonic lethality during organogenesis		√

Phenotypic
data by
genotype

Phenotypic Data by Genotype

(show or hide all phenotypic details)

Genotype	Allelic Composition	Genetic Background
hm1	Itga4^tm1Hyn/Itga4^tm1Hyn	either: (involves: 129S2/SvPas) or (involves: 129S2/SvPas * C57BL/6J)
lethality/prenatal-perinatal embryonic lethality during organogenesis (J:23140) about 50% die at around E11 with failure of allantois-chorion fusion about 50% die around E12.5 with cardiac defects, although a few survive up to E14.5 embryogenesis abnormal extraembryonic tissue morphology (J:23140) about half of the embryos exhibit allantois defects between E9.5 and E11.5 dilated allantois (J:23140) allantois has an abnormal balloon shape abnormal placenta morphology (J:23140) placenta lacks the allantoic component at E9.5 and as a result, the placenta does not form a connection with the embryo failure of chorioallantoic fusion (J:23140) failure of fusion of the allantois with the chorion cardiovascular system abnormal cardiovascular system morphology (J:23140) the 50% of embryos that survive past E11.5 develop cardiac abnormalities between E11.5 and E14.5 but do not have allantois defects abnormal coronary artery morphology (J:23140) coronary vessels at the atrioventricular sulcus region are absent abnormal epicardium morphology (J:23140) epicardium is missing on the entire ventricular surface and on part of the atrial surface at E11.5, but not at E10.5 heart hemorrhage (J:23140) cardiac hemorrhage craniofacial abnormal craniofacial morphology (J:23140) embryos with cardiac defects exhibit abnormalities in the cranial and facial structures
Genotype	Allelic Composition	Genetic Background
ht2	Itga4^tm1Hyn/Itga4^tm1Jty	involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6N
cardiovascular system abnormal epicardium morphology (J:76747) epicardium fails to form

References

Original:	J:23140 Yang JT et al., "Cell adhesion events mediated by alpha 4 integrins are essential in placental and cardiac development." Development 1995 Feb;121(2):549-60
All:	6 reference(s)

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