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Crhr1tm4.1(cre)Jde - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Crhr1tm4.1(cre)Jde
corticotropin releasing hormone receptor 1; targeted mutation 4.1, Jan Deussing 		Driver: Crhr1
Type: Targeted (Recombinase)
Synonym: Crhr1iCre, Crhr1-ires-Cre
Molecular description: Mice expressing Cre recombinase under the control of the Crhr1 promoter were generated by using a recombinase-mediated cassette exchange (RMCE) strategy. A construct with a docking site contained (5' to 3') an attP site, loxP site, FRT site, splice acceptor, T2A cleavage site, tau/lacZ/flag reporter cassette, FRT site, neo cassette, attP site, PGK promoter and FRT site, was inserted into intron 2. The attB site-flanked Crhr1-Cre recombinase expression unit encompassed, from 5' to 3', Crhr1 intron 2 to exon 3 fused to the Crhr1 cDNA covering exons 4-13, an ires-Cre cassette with a bGH-pA, and a frt site followed by a Pgk1 polyadenylation sequence and hygromycin resistance cassette, both in inverse orientation. phiC31 integrase-mediated cassette exchange resulted in insertion of the Crhr1-Cre expression unit into the right attP site. Resulting ES cells were used to generate chimeric mice, and mouse line Crhr1tm4Jde. The tau-lacZ reporter and hygromycin selection cassette were removed by breeding to FLPeR mice.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Crhr1tm4.1(cre)Jde activity also observed in:
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Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory