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Tg(Nes-phiC31*/ERT2)2Imayo - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Nes-phiC31*/ERT2)2Imayo
transgene insertion 2, Itaru Imayoshi
Driver: Nes
Type: Transgenic (Inducible, Recombinase)
Inducer: induced by tamoxifen
Synonym: Nestin-PhiC31-ERT2 line 2
Molecular description: The construct was composed of a 5.8 kb fragment of the nestin promoter with sequences for optimized phiC31 recombinase (phiC31*; coding sequence in this construct lacks the nuclear localization signal) fused to the tamoxifen-inducible mutated estrogen receptor sequence (ERT2), followed by the 1.8 kb nestin second intron containing the neural stem/progenitor cell-specific enhancer. The SV40 polyadenylation sequence was also cloned into the vector. The purified construct was injected into fertilized one-cell embryos. 7 founders were established with 2 lines (#2 and 4) chosen for further analysis based on high transgene expression in the ventricular zone of the developing nervous system.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/16/2014
MGI 5.19
The Jackson Laboratory