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Ccktm2.1(cre/ERT2)Zjh - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Ccktm2.1(cre/ERT2)Zjh
cholecystokinin; targeted mutation 2.1, Z Josh Huang
Driver: Cck
Type: Targeted (Inducible, Recombinase)
Inducer: induced by tamoxifen
Synonym: Cck-CreER
Molecular description: A targeting vector was designed to insert a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette. These Cck-CreER (or Cck-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for two additional generations and then bred together for many more generations (and the FLPe transgene was removed).
Collection: Neuroscience Blueprint cre
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/23/2014
MGI 5.19
The Jackson Laboratory