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Tg(Tmem100-EGFP/cre/ERT2)30Amc - Renal And Urinary System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Tmem100-EGFP/cre/ERT2)30Amc
transgene insertion 30, Andrew P McMahon
Driver: Tmem100
Type: Transgenic (Inducible, Recombinase, Reporter)
Inducer: induced by tamoxifen
Synonym: Tmem100-GCE, TmemGCE
Molecular description: A targeting construct containing the eGFPCreERT2 (GCE) cassette and Frt site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-183L17). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA)F1 donor eggs. Founder line 30 was subsequently established. Native GFP expression is detectable in the urogenital system of E15.5 embryos. Transgenic cre expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. Possible cre recombinase activity is detected in small renal arteries.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Tmem100-EGFP/cre/ERT2)30Amc activity also observed in:
cardiovascular system, reproductive system
Tissue Information
Renal And Urinary System Other recombinase alleles with activity in Renal And Urinary System tissues:
Images
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J:159099 Fig. 10 Tmem100 J:159099 Fig. 2 Tmem100 J:159099 Fig. 3 Tmem100 J:159099 Fig. 4 Tmem100 J:159099 Fig. 5 Tmem100 J:159099 Fig. 6 Tmem100 J:159099 Fig. 7 Tmem100 J:159099 Fig. 8 Tmem100
J:159099 Fig. 9 Tmem100
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/26/2014
MGI 5.19
The Jackson Laboratory