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Gad2tm1(cre/ERT2)Zjh - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Gad2tm1(cre/ERT2)Zjh
glutamic acid decarboxylase 2; targeted mutation 1, Z Josh Huang 		Driver: Gad2
Type: Targeted (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Gad2-CreER, Gad2-CreERT2, GAD65-CreER, GAD65-CreERT2
Molecular description: A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into 129 x C57BL/6 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with white C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette.
Collection: Neuroscience Blueprint cre
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Gad2tm1(cre/ERT2)Zjh activity also observed in:
head, sensory organs
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Images
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J:216865 Full Image
Fig. Gad2 Adult Hippocampus J:216865 Fig. Gad2 Adult hypo. by optic tract J:216865 Full Image
Fig. Gad2 Adult Hypothalamus J:216865 Full Image
Fig. Gad2 Adult Midbrain J:216865 Full Image
Fig. Gad2 Adult Spinal cord
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 15 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory