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Tg(Tbx18-icre)1Fech - Integumental System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Tbx18-icre)1Fech
transgene insertion 1, Feng Chen
Driver: Tbx18
Type: Transgenic (Cre/Flp)
Synonym: Tbx18-Cre, Tbx18creTg
Molecular description: A BAC clone (RP23-35307) carrying the entire mouse Tbx18 gene was recombineered such that the first exon was replaced with the coding sequence for codon-improved cre recombinase (icre) fused to a nuclear localization signial (NLS) and a Myc tag. An frt-flanked Amp(r) selection cassette followed the icre and was removed by transient Flp expression. The cre cassette carries a transcription termination signal so transcription from the Tbx18 promoter is terminated upstream of exon 2, effectively abolishing transcription of any Tbx18 sequence. The linearized construct was microinjected into fertilized oocytes from C57BL/6 x CBA hybrids. Three founders were obtained, crossed to R26R-LacZ reporter mice, and all showed similar reporter gene expression patterns, with line 2 having a lower level of cre activity. Lines 1 and 3 were used for further analysis.
Find mice (IMSR): Mouse Strains: 0 lines available       Cell Lines: 0 lines available
Additional Tissues: Tg(Tbx18-icre)1Fech activity also observed in:
branchial arches, cardiovascular system, limbs, mesenchyme, renal and urinary system, reproductive system
Tissue Information
Integumental System Other recombinase alleles with activity in Integumental System tissues:
Images
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J:150023 Fig. 4
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
04/08/2014
MGI 5.17
The Jackson Laboratory