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Cdkn2atm3(cre)Cjs - Reproductive System
Recombinase Activity Detail
Allele Information
Allele:
Cdkn2atm3(cre)Cjs
cyclin-dependent kinase inhibitor 2A; targeted mutation 3, Charles J Sherr
Driver: Cdkn2a
Type: Targeted (Recombinase)
Synonym: Arf-Cre
Molecular description: The targeting vector contains the sequence for cre recombinase and its 3' polyadenylation signal ligated in the opposite orientation to a dual selection cassette (Neo-Tk) containing a neomycin resistance gene and a thymidine kinase gene under control of their respective 5' promoters and polyadenylation. The selection cassette was flanked by Frt sites for deletion in ES cells. Homologous recombination left the Cdkn2a promoter and 5' UTR intact, but replaced most of exon 1Beta with cre cDNA. The linearized vector with a DTA selectable marker at the 5' end was electroporated into ES cells. It was determined that removal of the Neo-TK cassette by transfection of ES cells with FLP recombinase did not result in transmission of the cre knock-in allele, so the Neo-Tk was left intact in the positive chimeras.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Cdkn2atm3(cre)Cjs activity also observed in:
sensory organs
Tissue Information
Reproductive System Other recombinase alleles with activity in Reproductive System tissues:
Images
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J:147752 Fig. 1
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/09/2014
MGI 5.19
The Jackson Laboratory