A 2.5 kb fragment of the Cdh5 promoter region followed by the cre recombinase cDNA and the SV40 polyadenylation signal was inserted into the pBluescriptSKII plasmid. The transgene fragment was excised and microinjected into C57BL/6J fertilized oocytes. Three founder lines were obtained, All three showed no differences in reporter gene expression when crossed to EGFP or LacZ reporter mice.
Line 1 with an estimated 2 copies of the Cdh5-cre transgene was analyzed further.
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