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Tg(Ddx4-cre)1Dcas - Cardiovascular System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Ddx4-cre)1Dcas
transgene insertion 1, Diego H Castrillon 		Driver: Ddx4
Type: Transgenic (Null/knockout, Recombinase)
Synonym: Mvh-Cre, Vasa-Cre
Molecular description: A 5.6kb fragment of the Ddx4 promoter was ligated to the cre open reading frame with an engineered nuclear localization signal. Eight transgenic lines were created but only two possessed expressing in the gonads as early as E15. When the cre transgene is maternally expressed, all progeny can show ubiquitous recombination even if the cre is not inherited, likely due to perdurance of cre protein in the egg. The transgene integrated into chromosome 18 causing a 1098 Kb deletion and deleting the genes Neto1 and Cbln2. The deletion results in a functional knock-out of both genes in homozygous mice. Founder line 1 has a copy number of greater than 20.
Find mice (IMSR): Mouse Strains: 2 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Ddx4-cre)1Dcas activity also observed in:
Tissue Information
Cardiovascular System Other recombinase alleles with activity in Cardiovascular System tissues:
Images
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J:260776 Full Image
Fig. Ddx4 adult heart1 J:260776 Full Image
Fig. Ddx4 adult heart2 J:260776 Full Image
Fig. Ddx4 adult heart3 J:260776 Full Image
Fig. Ddx4 adult vessels1 J:260776 Full Image
Fig. Ddx4 adult vessels2 J:260776 Full Image
Fig. Ddx4 adult vessels3 J:260776 Full Image
Fig. Ddx4 E10.5 atrium J:260776 Full Image
Fig. Ddx4 E10.5 bulbous cordis
J:260776 Full Image
Fig. Ddx4 E10.5 dorsal aorta J:260776 Full Image
Fig. Ddx4 E10.5 endcardial cussion J:260776 Full Image
Fig. Ddx4 E10.5 sinus venosus J:260776 Full Image
Fig. Ddx4 E10.5 ventical J:260776 Full Image
Fig. Ddx4 E15.5 aorta J:260776 Full Image
Fig. Ddx4 E15.5 atria J:260776 Full Image
Fig. Ddx4 E15.5 interventricular septum J:260776 Full Image
Fig. Ddx4 E15.5 vena cava
J:260776 Full Image
Fig. Ddx4 E15.5 ventricle
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 172 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
04/09/2024
MGI 6.23 		The Jackson Laboratory