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pMT4 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pMT4
  • Sequence Type
    cDNA
  • ID
    MGI:10068
  • Region Covered
    full length cDNA
  • Child Clones
    MT4.Pv.25, pTRP1.6
  • Insert Size
    ~2.6 kb
Source
  • Species
    mouse, laboratory
  • Tissue
    melanocyte
  • Cell Line
    melanoma cell line B16
Genes
Tyrp1 tyrosinase-related protein 1
Sequences
X03687 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
Polymorphisms
J:9222 Jackson IJ, Proc Natl Acad Sci U S A. 1988 Jun;85(12):4392-6
Notes: A 256 bp PvuII (ntds 682-943) fragment and 1.6 kb HindIII fragment was isolated from pMT4 and used in Southern analysis. Additional bands picked up by this probe include 6.6, 3.2, and 2.65 kb fragments in all strains listed and a 12.8 and 9 kb band witha different strain distribution probably indicating an independent locus.
Endonuclease Gene Allele Fragments Strains
TaqI Tyrp1 b 3.7,1.2kb 101/H, 129, AKR/J, C3H/HeH, C57BL/6J, CBA/Ca, LP/J
d 4.9kb BALB/cBy, C57L/J, DBA/2J, NZW/LacJ, ST/bJ
J:10819 Bahary N, et al., Proc Natl Acad Sci U S A. 1990 Nov;87(21):8642-6
Notes: Probe name not given in this reference; inferred from the source and source reference for the probe. M.spretus vs. C57BL/6J-db/db alleles stated as differing also; endonuclease used and fragment sizes not specified.
Endonuclease Gene Allele Fragments Strains
TaqI Tyrp1 b absent C57BL/6J
d 4.9kb DBA/2J
J:3321 Bahary N, et al., Genomics. 1991 Sep;11(1):33-47
Endonuclease Gene Allele Fragments Strains
TaqI Tyrp1 b 12.8,6.6,3.7,3.3kb C57BL/6J
s 6.6,3.7,2.4,0.9kb M. spretus
J:11192 Fletcher C, et al., Genomics. 1991 Apr;9(4):647-55
Notes: Only differential fragments listed; additional bands visible on autoradiograms. A 1600 bp fragment of clone PMT4 used for Southern analysis.
Endonuclease Gene Allele Fragments Strains
RsaI Tyrp1 b absent BKS.Cg-mea2J Dock7m
c ~1.5,~3.0kb M. m. castaneus
J:12921 Smith CA, et al., Cell. 1993 Jul 2;73(7):1349-60
Endonuclease Gene Allele Fragments Strains
KpnI Tyrp1 b 2.5kb C57BL/6J
s 2.4kb M. spretus
References
J:8247 Shibahara S, et al., Cloning and expression of cDNA encoding mouse tyrosinase. Nucleic Acids Res. 1986 Mar 25;14(6):2413-27
J:9222 Jackson IJ, A cDNA encoding tyrosinase-related protein maps to the brown locus in mouse [published erratum appears in Proc Natl Acad Sci U S A 1989 Feb;86(3):997]. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4392-6
J:10819 Bahary N, et al., Molecular mapping of the mouse db mutation. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8642-6
J:3321 Bahary N, et al., Molecular genetic linkage maps of mouse chromosomes 4 and 6. Genomics. 1991 Sep;11(1):33-47
J:11192 Fletcher C, et al., Genetic mapping of meander tail, a mouse mutation affecting cerebellar development. Genomics. 1991 Apr;9(4):647-55
J:12921 Smith CA, et al., CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF. Cell. 1993 Jul 2;73(7):1349-60
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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