Previous Next

Differences Between C57BL Strains at an Erythrocyte Antigen Locus

Marianna Cherry
Donald W. Bailey
George D. Snell

The Jackson Laboratory
Bar Harbor, Maine

Three different strain combinations have produced alloantisera that agglutinate red cells from mouse strains C57BL/6J and C57BL/By, but do not react with cells from strains C57BL/10Sn, C57BL/10J, or C57BL/10Gn-Rn/+ (Rn/+ or +/+). Erythrocytes from 35 H-2 and non-H-2 congenic lines on the C57BL/Sn background are unreactive, whereas red cells from all other inbred strains tested to date, including related strains C57L/J, C57BR/cdJ, C57BL/KsJ, and C58/J are reactive. This pattern of serological reactivity is different from that of any known erythrocyte antigen system of the mouse and suggests residual heterozygosity or a mutation in an early progenitor of the C57BL/10 substrain family.

We have recently developed a congenic line in which a histocompatibility (H) allele from strain C57BL/10Sn was introduced onto a C57BL/6J background by 16 successive generations of backcross followed by sib mating. Cells from mice selected at the first intercross generation for homozygosity at the introduced H allele were serotyped with one of the alloantisera characterized above. They were unreactive or phenotypically like cells from strain C57BL/10Sn. Thus it appears that the erythrocyte antigen described here is determined by a locus closely linked to, or perhaps identical with, a histocompatibility locus that distinguishes between strains C57BL/6J and C57BL/10Sn.

Previous Next