En2
Gene Detail

Symbol Name ID

En2 engrailed 2 MGI:95390

Synonyms

En-2

Feature Type

protein coding gene

Genetic Map

Chromosome 5

13.94 cM
Detailed Genetic Map ± 1 cM


Mapping data(45)

Sequence Map

Chr5:28165696-28173612 bp, + strand From VEGA annotation of GRCm38   7917 bp   ±  kb flank VEGA Genome Browser | Ensembl Genome Browser | UCSC Browser | NCBI Map Viewer

Mouse Genome Browser

Vertebrate homology

HomoloGene:55579  Vertebrate Homology Class
1 human; 1 mouse; 1 rat; 1 chimpanzee; 1 rhesus macaque; 1 dog; 1 chicken; 2 zebrafish

Protein SuperFamily: homeobox protein, EN-1/EN-2 type
Gene Tree: En2

Human homologs

Human Homolog		EN2, engrailed homeobox 2
NCBI Gene ID		2020
neXtProt AC		NX_P19622
Human Chr (Location)		7q36; chr7:155250824-155257526 (+)  GRCh37.p10
Disease Associations		(1) Diseases Associated with Human EN2

Alleles and phenotypes

All alleles(165) : Targeted(11) Gene trapped(154)
 

This locus affects anterior-posterior cerebellar patterning. Homozygous null mutants show altered foliation pattern and perform poorly in motor learning (rotarod) tests. Heterozygotes test intermediate on rotarod. Hypomorphs show no phenotypic effects.

 
Human Diseases Modeled Using Mouse En2 (1)    Alleles Annotated to Human Diseases(1)   

Gene Ontology (GO) classifications

All GO classifications: (14 annotations)

Process	hindbrain development, midbrain development, ...
Component	membrane, nucleus
Function	DNA binding, sequence-specific DNA binding, ...

External Resources: FuncBase

Expression

Literature Summary: (129 records)
Data Summary: Results (632)    Tissues (209)    Images (118)
Theiler Stages: 10, 11, 12, 13, 14, 15, 16, 17, 19, 20, 21, 22, 23, 24, 25, 26, 28

Assay Type	Results
Immunohistochemistry	33
RNA in situ	426
Northern blot	31
RT-PCR	139
RNase protection	3

cDNA source data(17)
External Resources: Allen Institute   GENSAT   GEO   ArrayExpress

Molecular reagents

All nucleic(40) Genomic(6) cDNA(20) Primer pair(4) Other(10)
Microarray probesets(3)

Other database links

VEGA Gene Model	OTTMUSG00000037277 (Evidence)
Ensembl Gene Model	ENSMUSG00000039095 (Evidence)
Entrez Gene	13799 (Evidence)
UniGene	4298
DFCI	TC1587739
DoTS	DT.87072058, DT.91281408
NIA Mouse Gene Index	U005384
Consensus CDS Project	CCDS19143.1
International Mouse Knockout Project Status	En2

Sequences

Length	Strain/Species
genomic	OTTMUSG00000037277	VEGA Gene Model | MGI Sequence Detail	7917	C57BL/6J
transcript	OTTMUST00000096171	VEGA | MGI Sequence Detail	4983	Not Applicable
polypeptide	OTTMUSP00000053701	VEGA | MGI Sequence Detail	324	Not Applicable

All sequences(24) RefSeq(2) UniProt(2)

Polymorphisms

All PCR and RFLP(15) : PCR(1) RFLP(14) SNPs(10 from dbSNP Build 128)

Protein-related information

Resource	ID	Description
InterPro	IPR017970	Homeobox, conserved site
InterPro	IPR019549	Homeobox engrailed, C-terminal
InterPro	IPR019737	Homeobox engrailed-type, conserved site
InterPro	IPR001356	Homeodomain
InterPro	IPR000747	Homeodomain engrailed
InterPro	IPR009057	Homeodomain-like
InterPro	IPR020479	Homeodomain, metazoa
Protein Ontology	PR:000007071	homeobox protein engrailed-2

References

(Earliest) J:9012 Joyner AL, et al., En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis [published erratum appears in Genes Dev 1987 Jul;1(5):521]. Genes Dev. 1987 Mar;1(1):29-38
(Latest) J:193399 Park S, et al., GDE2 promotes neurogenesis by glycosylphosphatidylinositol-anchor cleavage of RECK. Science. 2013 Jan 18;339(6117):324-8
All references(235)

Other accession IDs

MGD-MRK-9498, MGD-MRK-9500, MGI:2141264