Tg(CAG-DMPK*)1323Coop
Transgene Detail

Summary

• Symbol: Tg(CAG-DMPK*)1323Coop
• Name: transgene insertion 1323, Thomas A Cooper
• MGI ID: MGI:5426792
• Synonyms: EpA960
• Transgene: Tg(CAG-DMPK*)1323Coop Location: unknown
• Alliance: Tg(CAG-DMPK*)1323Coop page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-DMPK*)1323Coop expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DMPK (1760)

  Dmpk	(MGI:94906)

• Mutation details: This transgene contains a ubiquitously expressed pCAGG CMV enhancer/chicken beta-actin promoter, a floxed concatemer of three SV40 polyadenylation sites, and the human DMPK exon 15 containing 960 copies of interrupted CTG repeats (containing CTCGA after every 20 CTG repeats). The SV40 polyadenylation sites prevent expression of RNA from downstream genomic segments, and their removal by Cre-mediated recombination results in transcription and splicing of DMPK exon 15 into the transgene mRNA. Three lines were made, with the 1323 line showing the highest expression of mRNA from the recombined allele. (J:127391)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:127391 Wang GS, et al., Elevation of RNA-binding protein CUGBP1 is an early event in an inducible heart-specific mouse model of myotonic dystrophy. J Clin Invest. 2007 Oct;117(10):2802-11
• All: 6 reference(s)