Tg(NPHS2-Trpc6)F419Walz
Transgene Detail

Summary

• Symbol: Tg(NPHS2-Trpc6)F419Walz
• Name: transgene insertion F419, Katherina Walz
• MGI ID: MGI:5319631
• Synonyms: Trpc6-HA wt
• Transgene: Tg(NPHS2-Trpc6)F419Walz Location: unknown
• Alliance: Tg(NPHS2-Trpc6)F419Walz page

Transgene origin

• Strain of Origin: C57BL/6J x CBA/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(NPHS2-Trpc6)F419Walz expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Trpc6 (MGI:109523)

• Mutation details: The Trpc6-HA wildtype cDNA sequence was made by modifying a Trpc6 cDNA sequence (encoding a 930 amino acid protein) originally isolated from mouse brain tissue by both repairing an A2615G point mutation (D872G) and also by in-frame addition of an influenza virus hemaglutinin epitope (HA-tag) at the 3' end. The cDNA sequence was placed downstream of a 2.5 kbp fragment from the promoter/enhancer region of the human podocin locus (NPHS2) and upstream from a bovine growth hormone polyA sequence. Line F419 contains 2-4 copies of the transgene. (J:165112)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:165112 Krall P, et al., Podocyte-specific overexpression of wild type or mutant trpc6 in mice is sufficient to cause glomerular disease. PLoS One. 2010;5(9):e12859
• All: 1 reference(s)