Tg(UBC-Xrcc4,-GFP)1Jpdv
Transgene Detail

Summary

• Symbol: Tg(UBC-Xrcc4,-GFP)1Jpdv
• Name: transgene insertion 1, Jean-Pierre de Villartay
• MGI ID: MGI:5314247
• Synonyms: Tg(UBC-Xrcc4-TAP-iGFP), X4T
• Transgene: Tg(UBC-Xrcc4,-GFP)1Jpdv Location: unknown Genetic Position: Chr16, Syntenic
• Alliance: Tg(UBC-Xrcc4,-GFP)1Jpdv page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(UBC-Xrcc4,-GFP)1Jpdv expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Xrcc4 (MGI:1333799)

• Mutation details: This transgene was produced by transfection of single-cell embryos by a self-inactivating lentiviral vector with a truncated 3' LTR into which a loxP site has been introduced; upon integration, the 3' LTR is duplicated at the 5' end of the insertion so that the entire transgene can be excised by Cre recombinase, leaving behind a single loxP-delta-3' LTR. The vector contains the human ubiquitin C promoter upstream of the mouse Xrcc4 (X-ray repair complementing defective repair in Chinese hamster cells 4) open reading frame joined, in-frame, to a DNA fragment encoding a tandem affinity purification (TAP) tag -- which consists of a calmodulin binding peptide joined via a linker sequence containing a TEV protease cleavage site to two copies of the protein A IgG binding domain -- followed by an internal ribosomal entry site (IRES2) and the coding sequence for green fluorescent protein. Southern blot analysis demonstrated the presence of a single proviral integration, which was mapped by inverse PCR analysis to Chr 16, in the third intron of the Spag6 (sperm associated antigen 6) gene. Expression of the XRCC4 protein in spleen and thymus was demonstrated by immunoblot analysis, and the transgene's complementation of the embryonic lethal brain neuronal apoptosis phenotype of Xrcc4^tm1Alt homozygous mice demonstrates XRCC4 expression in the brain; GFP is not expressed in mice with this transgene. (J:125880, J:181755)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The authors "selected one chimera with a single integration to derive the X4 transgenic (X4T) line." (J:125880)

References

• Original: J:125880 Soulas-Sprauel P, et al., Role for DNA repair factor XRCC4 in immunoglobulin class switch recombination. J Exp Med. 2007 Jul 9;204(7):1717-27
• All: 2 reference(s)