Tg(Myh7-Pln)2Egk
Transgene Detail

Summary

• Symbol: Tg(Myh7-Pln)2Egk
• Name: transgene insertion 2, Evangelia G Kranias
• MGI ID: MGI:3834218
• Synonyms: Pln^OE, Tg(Myh6-Pln)2Egk, Tg(Pln)2Egk
• Transgene: Tg(Myh7-Pln)2Egk Location: unknown
• Alliance: Tg(Myh7-Pln)2Egk page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh7-Pln)2Egk expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pln (MGI:97622)

• Mutation details: This transgene contains the promoter of the mouse beta myosin heavy chain (myosin, heavy polypeptide 7, cardiac muscle, beta) gene, in a ~5.6 kb genomic DNA fragment encompassing upstream sequence and the entire 5-untranslated region (5-UTR) to the translation initiation site in exon 4, driving expression of a 603-bp mouse phospholamban cDNA fragment including 13 bp of the 5-UTR, the full-length protein coding sequence, and 434 bp of the 3-UTR, which is followed by the human growth hormone polyadenylation signal. Immunohistofluorescent analysis of transgenic soleus muscle demonstrated co-localization of PLN and sarcoplasmic reticulum (SR) Ca<2+>-ATPase (SERCA2a), which is responsible for SR Ca<2+> transport in cardiac and slow-twitch (Type I) skeletal muscle fibers. Whereas PLN was undetectable by western blot analysis of wild-type soleas muscle, in transgenic soleas its level relative to that of calsequestrin was ~90% of that observed in cardiac muscle, and the ratio of PLN to SERCA2a was similar between wild-type cardiac and transgenic soleas muscle. (J:127873)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:127873 Song Q, et al., Overexpression of phospholamban in slow-twitch skeletal muscle is associated with depressed contractile function and muscle remodeling. FASEB J. 2004 Jun;18(9):974-6
• All: 5 reference(s)