Tg(GFAP-B2m,GFAP-H2-K^b)1Gjh
Transgene Detail

Summary

• Symbol: Tg(GFAP-B2m,GFAP-H2-K^b)1Gjh
• Name: transgene insertion 1, Gunter J Hammerling
• MGI ID: MGI:3805042
• Synonyms: GFAP-K^b transgene, Tg(GFAP-H2-K^b)1Gjh
• Transgene: Tg(GFAP-B2m,GFAP-H2-K^b)1Gjh Location: unknown
• Alliance: Tg(GFAP-B2m,GFAP-H2-K^b)1Gjh page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1 x DBA/2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(GFAP-B2m,GFAP-H2-K^b)1Gjh expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	B2m (MGI:88127)
  mouse	H2-K (MGI:3040519)

• Mutation details: Two transgene constructs were co-injected, each containing the promoter of the glial fibrillary acidic protein (GFAP) gene upstream of DNA encoding either the mouse H2-K^b (MHC class I) molecule or mouse beta-2 microglobulin. Southern blot analysis demonstrates integration of both constructs on the same chromosome. Hybridization of allele-specific H2-K probes to reverse transcription- (RT-) PCR products detects H2-K^b transcripts only in brain, despite expression in other tissues of H2-K^d mRNA from the endogenous gene. H2-K allele-specific antibodies detect the H2-K^b protein on astrocytes and oligodendrocytes in cerebellar-cell culture. Immunohistologic analysis of brain sections detects H2-K^b in ependymal cells and in some choroid plexus epithelial cells. Immunohistologic examination of other sectioned organs finds H2-K^b only in intestine, specifically in Schwann cells of autonomic ganglia in the intestinal wall. No H2-K^b mRNA or protein is expressed in thymus. (J:134775)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Mice expressing this transgene in an H2^d background are immunologically tolerant of H2-K^b-expressing cells.

References

• Original: J:134775 Schonrich G, et al., Down-regulation of T cell receptors on self-reactive T cells as a novel mechanism for extrathymic tolerance induction. Cell. 1991 Apr 19;65(2):293-304
• All: 3 reference(s)