Tg(ACTB-EYFP/TUBA)1Paw
Transgene Detail

Summary

• Symbol: Tg(ACTB-EYFP/TUBA)1Paw
• Name: transgene insertion 1, Anthony Pawson
• MGI ID: MGI:3624422
• Synonyms: EYFP-tubulin transgene
• Transgene: Tg(ACTB-EYFP/TUBA)1Paw Location: unknown
• Alliance: Tg(ACTB-EYFP/TUBA)1Paw page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:100110
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene was constructed by joining the coding sequence and SV40 polyadenylation signal from the p-EYFP-Tub vector (Clontech), which encodes a fusion protein composed of enhanced yellow fluorescent protein (EYFP) and a human alpha-tubulin, downstream of the chicken beta-actin promoter followed by a Pgk-puromycin resistance cassette. Mitotic spindles of regenerating hepatocytes from partially hepatectomized mice bearing this transgene fluoresce brightly. (J:100110)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The linearized transgene construct was electroporated into ES cells. Tetraploid aggregation embryos derived from an ES cell clone designated Tub2 were completely fluorescent. Diploid aggregation using this clone generated chimeric mice from which a colony of transgenic mice was derived.

References

• Original: J:100110 Ko MA, et al., Plk4 haploinsufficiency causes mitotic infidelity and carcinogenesis. Nat Genet. 2005 Aug;37(8):883-8
• All: 1 reference(s)