Tg(LIPC*S145G)1Ssf
Transgene Detail
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Symbol: |
Tg(LIPC*S145G)1Ssf |
Name: |
transgene insertion 1, Silvia Santamarina-Fojo |
MGI ID: |
MGI:3620047 |
Synonyms: |
HL-S145G |
Transgene: |
Tg(LIPC*S145G)1Ssf Location: unknown
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Alliance: |
Tg(LIPC*S145G)1Ssf page
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Transgene Type: |
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Transgenic (Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(LIPC*S145G)1Ssf expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
LIPC (3990) |
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Mutation details: The transgene comprises a cDNA encoding the enzymatically nonfunctional S145G mutant human hepatic lipase (uniprot E7EUK6; NM_000236.3:c.616A>G;p.Ser206Gly) cloned between the promoter and polyadenylation signal of the human apolipoprotein E (APOE) gene; it also includes the hepatic and macrophage control regions from the Apoe/Apoc1 locus. Northern blot analysis demonstrated expression of the transgenic mRNA in liver, but not in any of 8 other organs/tissues examined. Western blot analysis revealed presence of the human protein in post-heparin plasma. The level of both mRNA and protein expression is 3- to 5-fold that of endogenous gene expression in wild-type mice.
(J:94495)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Mutant human hepatic lipase lacking catalytic function but capable of binding ligand is expressed specifically in the liver.
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Original: |
J:94495 Gonzalez-Navarro H, et al., The ligand-binding function of hepatic lipase modulates the development of atherosclerosis in transgenic mice. J Biol Chem. 2004 Oct 29;279(44):45312-21 |
All: |
2 reference(s) |
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